Effect of insulin on ATP-citrate lyase phosphorylation: regulation of peptide A and peptide B phosphorylations.
Effect of insulin on ATP-citrate lyase phosphorylation: regulation of peptide A and peptide B phosphorylations.
复制标题
胰岛素对 ATP-柠檬酸裂解酶磷酸化的影响：肽 A 和肽 B 磷酸化的调节。
DOI：
10.1021/bi00428a067
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发表时间：
1989
期刊：
影响因子：
2.9
通讯作者：
Benjamin,WB
中科院分区：
文献类型：
--
作者：
Ramakrishna,S;Murthy,KS;Benjamin,WB
材料与方法DEAE-纤维素DE-52购自Whatman。化学品的来源如所述（Ramakrishna和Benjamin，1988年）。脂肪垫与激素的孵育。将来自100-125-g雄性Sprague-Dawley大鼠（Taconic Farms，Germatown，NY）的附睾脂肪垫在含有3%BSA和5 mM葡萄糖的改良Krebs-Ringer Hepes缓冲液（pH 7.4）中在37 ℃下孵育，如先前所述（Benjamin & Singer，1975）。[32P]在一些烧瓶中加入P10，继续孵育90分钟（Ramakrishna & Benjamin，1984）。胰岛素（100 nM）或异丙肾上腺素（1 µ）1缩略语：Hepes，4-（2-羟乙基）-1-哌嗪乙磺酸; PMSF，苯甲磺酰氟; TLCK，N-（对甲苯磺酰基）-L-赖氨酸氯甲基酮; SDS，十二烷基硫酸钠; PAGE，聚丙烯酰胺凝胶电泳; PEG，聚（乙二醇）; MFPK，多功能蛋白激酶; A-激酶，cAMP依赖性蛋白激酶; ATP-CL，ATP-柠檬酸裂解酶。
Materials and MethodsMaterials. DEAE-cellulose DE-52 was purchased from Whatman. The sources of chemicals were as described (Ramakrishna & Benjamin, 1988). Incubation of Fat Pads with Hormones. Epididymal fat pads from 100-125-g male Sprague-Dawley rats (Taconic Farms, Germatown, NY) were incubated in modified Krebs-Ringer Hepes buffer, pH 7.4, containing 3% BSA and 5 mM glucose at 37 C as previously described (Benjamin & Singer, 1975).[32P] P¡ was added to some flasks, and the incubations were continued for 90 min (Ramakrishna & Benjamin, 1984). Insulin (100 nM) or isoproterenol (1 µ)1 Abbreviations: Hepes, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; PMSF, phenylmethanesulfonyl fluoride; TLCK, N-(j>-tosyl)-L-lysine chloromethyl ketone; SDS, Sodium dodecyl sulfate; PAGE, polyacrylamide gel electrophoresis; PEG, poly (ethyleneglycol); MFPK, multifunctional protein kinase; A-kinase, cAMP-dependent protein ki-nase; ATP-CL, ATP-citrate lyase.