Improvement of proline chiral stationary phases by varying peptide length and linker.
Improvement of proline chiral stationary phases by varying peptide length and linker.
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DOI：
10.1016/j.chroma.2006.01.128
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发表时间：
2006-04
期刊：
影响因子：
--
通讯作者：
Junmin Huang;Hui Chen;Tingyu Li
中科院分区：
文献类型：
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作者：
Junmin Huang;Hui Chen;Tingyu Li
为了改善手性固定相的性能，制备并评价了7种不同游离基团数目和/或不同硅胶键合的新型手性固定相。53种分析物的平均分离因子随着固定相中的脯氨酸单元数目的增加而增加。当不使用6-甲氨基己酸连接物时，将脯氨酸肽直接连接到3-甲氨基丙基硅胶上时，固定相的整体性能更好。对于手性固定相8，分离效果还取决于所使用的流动相体系。对于该固定相，CH2Cl2/己烷/异丙醇体系的性能明显优于异丙醇/己烷体系。对于所测试的53种分析物，该固定相实现的分离因子与三种商业色谱柱的分离因子相当。
Seven new stationary phases with different number of proline units and/or different linkage to silica gel were prepared and evaluated in order to improve the performance of proline chiral stationary phases. The average separation factor achieved with the 53 analytes increases with the number of proline units in the stationary phases. When the proline peptides are directly attached to the 3-methylaminopropyl silica gel without using the 6-methylaminohexanoic acid linker, the stationary phases perform better overall. For decaproline chiral stationary phase 8, the separation also depends on the mobile phase system used. For this stationary phase, the CH2Cl2/hexanes/2-propanol system significantly outperforms the 2-propanol/hexanes system. For the 53 analytes tested, the separation factors achieved with this stationary phase compare well with those for three commercial columns.