Stability of chitin synthetase in cell-free preparations of a wild-type strain and a 'slime' variant of Neurospora crassa.
Stability of chitin synthetase in cell-free preparations of a wild-type strain and a 'slime' variant of Neurospora crassa.
复制标题
粗糙脉孢菌野生型菌株和“粘液”变体的无细胞制剂中几丁质合成酶的稳定性。
DOI：
10.1111/j.1574-6968.1991.tb04436.x-i1
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发表时间：
1991
影响因子：
2.1
通讯作者：
Martínez,JP
中科院分区：
文献类型：
--
作者：
Giménez,G;Gozalbo,D;Martínez,JP
在0°C下储存的样品中，对来自粗糙脉孢菌野生型菌株和“粘液”变体的无细胞制剂中的几丁质合成酶活性进行了多天的监测。两种生物体的全细胞无提取物和低速上清液中的总活性非常不稳定，在0°C下储存96 h时损失超过90%的初始活性。几丁质合成酶检测没有被制剂中存在的几丁质酶活性所掩盖。这些制剂的凝胶过滤色谱法增加了活性的稳定性，从“粘液”的变体，而通过高速离心去除颗粒结构稳定的几丁质合成酶活性的上清液中，特别是在野生型。这些结果表明，参与几丁质合成酶调节的因子在粘液细胞中可能存在差异或改变。
Chitin synthetase activity in cell-free preparations from a wild-type strain and a ‘slime’ variant ofNeurospora crassawas monitored over many days in samples stored at 0°C. Total activity in whole-cell-free extracts and low-speed supernatants from both organisms was very unstable, losing more than 90% of the initial activity on storage at 0°C for 96 h. Chitin synthetase detection was not masked by chitinase activity present in the preparations. Gel-filtration chromatography of these preparations increased the stability of the activity from the ‘slime’ variant, whereas removal of particulate structures by high-speed centrifugation stabilized the chitin synthetase activity in the supernatant, particulary in the wild type. These results suggest that factor(s) involved in the regulation of chitin synthetase may be differentially located or altered in ‘slime’ cells.