THE KINESIN-LIKE PROTEIN KLP61F IS ESSENTIAL FOR MITOSIS IN DROSOPHILA
THE KINESIN-LIKE PROTEIN KLP61F IS ESSENTIAL FOR MITOSIS IN DROSOPHILA
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DOI：
10.1083/jcb.123.3.665
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发表时间：
1993-11-01
影响因子：
7.8
通讯作者：
GOLDSTEIN, LSB
中科院分区：
文献类型：
--
作者：
HECK, MMS;PEREIRA, A;GOLDSTEIN, LSB
我们在这里报告，最近发现的驱动蛋白样蛋白在果蝇，KLP 61 F，在有丝分裂突变致命的有机体的结果中断。我们发现，在KLP 61 F功能的情况下，纺锤体两极未能分离，导致单极有丝分裂纺锤体的形成。由此产生的多倍体细胞的中期停滞的表型是令人想起在真菌bimC和cut7突变中看到的，其中也已显示纺锤体极体不分离。KLP 61 F在胚胎和幼虫发育期间的增殖组织中特异性表达，与细胞分裂中的主要作用一致。KLP 61 F，bimC，cut7，和Eg5驱动蛋白样蛋白的结构和功能的同源性表明存在一个保守的家族的驱动蛋白样分子重要的纺锤体极分离和有丝分裂纺锤体动力学。
We report here that disruption of a recently discovered kinesin-like protein in Drosophila melanogaster, KLP61F, results in a mitotic mutation lethal to the organism. We show that in the absence of KLP61F function, spindle poles fail to separate, resulting in the formation of monopolar mitotic spindles. The resulting phenotype of metaphase arrest with polyploid cells is reminiscent of that seen in the fungal bimC and cut7 mutations, where it has also been shown that spindle pole bodies are not segregated. KLP61F is specifically expressed in proliferating tissues during embryonic and larval development, consistent with a primary role in cell division. The structural and functional homology of the KLP61F, bimC, cut7, and Eg5 kinesin-like proteins demonstrates the existence of a conserved family of kinesin-like molecules important for spindle pole separation and mitotic spindle dynamics.