Trinitrophenol reactive T-cell hybridomas recognize antigens that require antigen processing.
Trinitrophenol reactive T-cell hybridomas recognize antigens that require antigen processing.
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三硝基苯酚反应性 T 细胞杂交瘤可识别需要抗原加工的抗原。
DOI：
10.1111/1523-1747.ep12389535
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Bigby,M
中科院分区：
文献类型：
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作者：
Ma,J;Wang,JH;Sy,MS;Guo,YJ;Hauser,C;Bigby,M
在被T细胞识别之前，蛋白质抗原必须与主要组织相容性复合体分子结合，被摄取、加工并展示在抗原提呈细胞的表面。用于研究小鼠过敏性接触过敏反应的半抗原的识别是否同样需要处理，目前还没有确定。我们通过研究抗原处理和提呈抑制剂对同基因B细胞瘤(A20)向一系列产生白介素2、三硝酚特异性、主要组织相容性复合体II类限制性T细胞杂交瘤呈递三硝基苯酚的能力的影响，分析了三硝基苯酚呈递给三硝基苯酚反应性T细胞杂交瘤是否需要抗原处理。三硝基苯酚修饰的A20细胞刺激杂交瘤的能力被非克隆抗体、抗三硝基酚抗体或抗Ia抗体完全抑制，并在三硝基苯酚修饰后立即被多聚甲醛固定显著降低。三硝基苯酚修饰的A20在固定前在37℃培养2h对杂交瘤细胞的刺激作用明显高于三硝基苯酚修饰的A20，因为氯喹抑制了三硝基苯酚的存在。多聚甲醛固定和氯喹处理对三硝基苯酚修饰的淋巴结树突状细胞刺激三硝酚特异性杂交瘤的作用相似。多聚甲醛固定和氯喹处理对A20细胞呈递卵白蛋白到卵清蛋白特异性杂交瘤的能力的影响与它们对三硝基苯酚修饰的A20细胞呈递三硝基苯酚到三硝基苯酚特异性杂交瘤的能力的影响相似。七个T细胞杂交瘤中有一个对三硝基苯酚修饰的卵清蛋白有反应，但对其他三硝基苯酚修饰的蛋白没有反应。这些结果表明，至少在一定程度上，对三硝基苯酚的接触性超敏反应中的T细胞识别需要处理的抗原，并且三硝基苯酚修饰的蛋白质可以被识别。
Protein antigens must be taken up, processed, and displayed on the surface of antigen-presenting cells in association with major histocompatibility complex molecules before they can be recognized by T cells. Whether recognition of the haptens used to study allergic contact hypersensitivity in murine models similarly requires processing has not been determined. We analyzed whether presentation of trinitrophenol to trinitrophenol reactive T-cell hybridomas requires antigen processing by studying the effects of inhibitors of antigen processing and presentation on tile ability of a syngeneic B-cell tumor (A20) to present trinitrophenol to a series of interleukin-2 producing, trinitrophenol specific, major histocompatibility complex class II-restricted T-cell hybridomas.The ability of trinitrophenol modified A20 cells to stimulate the hybridomas was completely inhibited by rnonoclonal, anti-trinitrophenol, or anti-Ia antibodies and was significantly reduced by paraformaldehyde fixation immediately after trinitrophenol modification. Trinitrophenol-modified A20 cultured at 37°C for 2 h prior to fixation was significantly more effective at stimulating the hybridomas than trinitrophenol-modified A20 to present trinitrophenol was inhibited by chloroquine. Paraformaldehyde fixation and chloroquine treatment had similar effects on the ability of trinitrophenol modified lymph node dendritic cells to stimulate the trinitrophenol specific hybridomas. Paraformaldehyde fixation and chloroquine treatment had similar effects on the ability of A20 cells to present ovalbumin to ovalbumin-specific hybridomas as they had on the ability of trinitrophenol modified A20 cells to present trinitrophenol to the trinitrophenol specific hybridomas. One of seven T-cell hybridomas responded to trinitrophenol modified ovalbumin but not other trinitrophenol modified proteins. These results suggest that, at least in part, T cells in the contact hypersensitivity response to trinitrophenol recognize antigens that require processing and that trinitrophenol modified proteins can be recognized.