Cystometric parameters and the activity of signaling proteins in association with the compensation or decompensation of bladder function in an animal experimental model of partial bladder outlet obstruction
Cystometric parameters and the activity of signaling proteins in association with the compensation or decompensation of bladder function in an animal experimental model of partial bladder outlet obstruction
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DOI：
10.3892/ijmm.2013.1515
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发表时间：
2013-12-01
影响因子：
5.4
通讯作者：
Lee, Tack
中科院分区：
文献类型：
--
作者：
Choi, Bo-Hwa;Jin, Long-Hu;Lee, Tack
我们进行了这项研究，以确定逼尿肌收缩的程度是否与膀胱功能代偿或失代偿有关，这取决于膀胱出口部分梗阻（BOO）发病后最初两周内的残留容积（RV）。此外，我们还研究了信号蛋白[amp活化激酶（AMPK），细胞外信号调节蛋白激酶1和2 （ERK1/2）和蛋白激酶C (PKC)]的磷酸化和表达程度是否与膀胱功能代偿或失代偿的患病率相关。选取雌性SD大鼠27只，随机分为假手术组（n=7）和膀胱出口部分梗阻组（n=20）。然后，我们从三个可重复的排尿周期中测量了膀胱计量参数，并将结果平均值作为两组之间的比较。基于平均RV%为25%的临界值，我们将实验动物细分为两个亚组：膀胱代偿亚组（平均RV%为25%）。我们的研究结果表明，在部分性BOO动物实验模型中，在BOO发病后的头两周内，逼尿肌过度活动（DO）的程度与膀胱功能的代偿或失代偿相关，这取决于RV。此外，我们还证明AMPK和ERK1/2参与膀胱功能的代偿或失代偿。此外，我们的结果表明PKC与两期膀胱收缩无关。信号蛋白如AMPK和ERK1/2活性的改变可能有助于排尿困难患者的治疗。
We conducted this study to determine whether the degree of detrusor contractility is associated with the compensation or decompensation of bladder function depending on the residual volume (RV) during the first two weeks after the onset of partial bladder outlet obstruction (BOO). Moreover, we also examined whether the degree of the phosphorylation and expression of signaling proteins [AMP-activated kinase (AMPK), extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) and protein kinase C (PKC)] is associated with the prevalence of compensation or decompensation of bladder function. Twenty-seven female Sprague-Dawley (SD) rats were randomly assigned to either the sham-operated group (n=7) or the group with partial bladder outlet obstruction (BOO) (n=20). We then measured cystometric parameters from three reproducible micturition cycles and averaged the results for a comparison between the two groups. Based on a cut-off value of a mean RV% of 25%, we subdivided our experimental animals into two subgroups: the subgroup with bladder compensation (mean RV%, 25%). Our results indicated that the degree of detrusor overactivity (DO) was associated with the compensation or decompensation of bladder function depending on the RV during the first two weeks after the onset of BOO in an animal experimental model of partial BOO. Moreover, we also demonstrate that AMPK and ERK1/2 are involved in the compensation or decompensation of bladder function. Furthermore, our results suggest that PKC is not involved in two-phase bladder contraction. Alterations in the activities of signaling proteins, such as AMPK and ERK1/2 may prove to be helpful in the treatment of patients with voiding difficulty.