VARICELLA-ZOSTER VIRUS - ISOLATION AND PROPAGATION IN HUMAN MELANOMA CELLS AT 36 AND 32-DEGREES-C
VARICELLA-ZOSTER VIRUS - ISOLATION AND PROPAGATION IN HUMAN MELANOMA CELLS AT 36 AND 32-DEGREES-C
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DOI：
10.1128/iai.19.1.199-203.1978
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发表时间：
1978-01-01
影响因子：
3.1
通讯作者：
BRUNELL, PA
中科院分区：
文献类型：
--
作者：
GROSE, C;BRUNELL, PA
来源于人恶性黑色素瘤的细胞系对水痘带状疱疹病毒（VZV）感染敏感。在接种囊泡液后5天内，在36 ℃温育的黑素瘤细胞单层培养物中出现细胞病变。或32 °。C.通过胰蛋白酶分散的感染细胞传代，在2种温度下连续繁殖VZV分离株。利用覆盖有含羧甲基纤维素的营养培养基的黑素瘤细胞单层开发空斑试验。通过该测定方法，VZV分离株在36 ℃下繁殖的生长周期为：C进行了研究，并与另一种在32 ℃下生长的VZV分离株进行了比较。C.以1个接种细胞对8个未感染细胞的比例使用等效的感染细胞接种物，在32 ℃的温育温度下无细胞病毒的产量为100%。C略高于36 ℃。C，虽然高峰出现在60小时，而不是36小时，感染后。低传代VZV的滴度在36 ℃下繁殖。当在32 ℃下测定时，C高0.5-1 log。而不是36度。C.
Cell lines derived from human malignant melanoma tumors are susceptible to infection with varicella-zoster virus (VZV). Within 5 days after inoculation of vesicular fluid, cytopathic changes appeared in melanoma cell monolayer cultures that were incubated at 36.degree. or 32.degree. C. The VZV isolates at the 2 temperatures were serially propagated by passage of trypsin-dispersed infected cells. A plaque assay was developed utilizing melanoma cell monolayers overlaid with nutrient medium containing carboxymethylcellulose. By this assay method, the growth cycle of a VZV isolate propagated at 36.degree. C was studied and compared with that of another VZV isolate grown at 32.degree. C. With equivalent infected-cell inocula at a ratio of 1 inoculum cell to 8 uninfected cells, the yield of cell-free virus at an incubation temperature of 32.degree. C was slightly higher than at 36.degree. C, although the peak occurred 60 h, rather than 36 h, postinfection. The titer of low-passage VZV propagated at 36.degree. C was 0.5-1 log higher when assayed at 32.degree. C rather than at 36.degree. C.