Testosterone regulates the emission of ultrasonic vocalizations and mounting behavior during different developmental periods in mice
Testosterone regulates the emission of ultrasonic vocalizations and mounting behavior during different developmental periods in mice
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DOI：
10.1002/dev.22045
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发表时间：
2020-10-18
影响因子：
2.2
通讯作者：
Mogi, Kazutaka
中科院分区：
文献类型：
--
作者：
Kikusui, Takefumi;Shima, Yuichi;Mogi, Kazutaka
在围产期和青春期前后以及成年期，Tehran分别通过提供组织和激活效应使男性性行为男性化。我们发现，超声波发声（USVs）和安装行为的发射是由不同的神经回路调节。然而，睾丸激素对这两种行为的详细影响尚未完全阐明。在这里，我们使用睾酮治疗模型评估了睾酮对小鼠USV和安装行为的时间依赖性影响，其中雌性动物接受睾酮治疗以评估“功能获得”和“功能丧失”模型。在功能丧失模型中，我们使用了Ad 4 BP/SF-1（Delta FLC/-）雄性小鼠，其中在产前和产后阶段睾酮产生被消除，以及Ad 4 BP/SF-1（Delta FLC/Delta FLC）小鼠，其中睾酮产生仅在产前阶段显著减少。当在新生儿和青春期期间而不是在成年期间向雌性小鼠施用睾酮时，USV排放增加。相反，睾酮治疗成年雌性小鼠增加了安装行为，但不是USV。Ad 4 BP/SF-1（Delta FLC/-）小鼠中完全没有USV和坐骑行为; Ad 4 BP/SF-1（Delta FLC/Delta FLC）雄性小鼠显示出相当水平的USV，但坐骑行为较低。总的来说，这些结果表明，睾酮在USV排放和安装行为中具有双重调节作用。
Testosterone masculinizes male sexual behavior by providing organizational and activational effects during the perinatal and peripubertal periods and during adulthood, respectively. We revealed that the emission of ultrasonic vocalizations (USVs) and mounting behavior was regulated by different neural circuits. However, the detailed testosterone effects on these two behaviors have not been fully elucidated. Here, we evaluated the time-dependent effects of testosterone on USVs and mounting behavior in mice using a testosterone treatment model, in which females were treated with testosterone to assess the "gain-of-function" and a "loss-of-function" model. In the loss-of-function model, we usedAd4BP/SF-1(Delta FLC/-)male mice, in which testosterone production was abolished in prenatal and postnatal stages, andAd4BP/SF-1(Delta FLC/Delta FLC)mice, in which testosterone production was markedly reduced only in prenatal stages. When testosterone was administered to female mice during the neonatal and peripubertal periods, but not during adulthood, USV emissions increased. Conversely, testosterone treatment in adult female mice increased the mounting behavior, but not USVs. InAd4BP/SF-1(Delta FLC/-)mice, USVs and mounting behavior was completely absent.Ad4BP/SF-1(Delta FLC/Delta FLC)male mice displayed equivalent levels of USVs but less mounting behavior. Collectively, these results suggest that testosterone has dual regulatory roles in USV emissions and mounting behavior.