Mutation of OsGIGANTEA Leads to Enhanced Tolerance to Polyethylene Glycol-Generated Osmotic Stress in Rice.
Mutation of OsGIGANTEA Leads to Enhanced Tolerance to Polyethylene Glycol-Generated Osmotic Stress in Rice.
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OsGIGANTEA 突变导致水稻对聚乙二醇产生的渗透胁迫的耐受性增强
DOI：
10.3389/fpls.2016.00465
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发表时间：
2016
影响因子：
5.6
通讯作者：
Shou H
中科院分区：
文献类型：
--
作者：
Li S;Yue W;Wang M;Qiu W;Zhou L;Shou H
水分亏缺是限制植物生长和作物产量的重要环境胁迫之一。虽然对植物缺水反应中涉及的许多关键因素的识别大大增加了我们对调节系统的了解，但植物脱水耐受性的机制仍然没有得到很好的理解。在本研究中，我们研究了关键开花时间调节剂OsGIGANTEA（OsGI）在水稻渗透胁迫耐受性中的作用。结果表明，OsGI基因的突变可提高水稻叶片对聚乙二醇（PEG）渗透胁迫的耐受性，增加脯氨酸和蔗糖含量，促进气孔运动。此外，qRT-PCR和基因芯片分析表明，OsDREB 1 E、OsAP 37、OsAP 59、OsLIP 9、OsLEA 3、OsRAB 16 A和OsSalT等渗透胁迫响应基因在osgi中的转录丰度显著高于野生型，表明OsGI可能是水稻渗透胁迫响应的负调控因子。
Water deficit is one of the most important environmental stresses limiting plant growth and crop yield. While the identification of many key factors involved in the plant water deficit response has greatly increased our knowledge about the regulation system, the mechanisms underlying dehydration tolerance in plants are still not well understood. In our current study, we investigated the roles of the key flowering time regulator, OsGIGANTEA (OsGI), in the osmotic stress tolerance in rice. Results showed that mutation of OsGI conferred tolerance to osmotic stress generated by polyethylene glycol (PEG), increased proline and sucrose contents, and accelerated stomata movement. In addition, qRT-PCR and microarray analysis revealed that the transcript abundance of some osmotic stress response genes, such as OsDREB1E, OsAP37, OsAP59, OsLIP9, OsLEA3, OsRAB16A, and OsSalT, was significantly higher in osgi than in WT plants, suggesting that OsGI might be a negative regulator in the osmotic stress response in rice.