DRUG-RESISTANCE AND BROAD GEOGRAPHICAL-DISTRIBUTION OF IDENTICAL R-PLASMIDS OF PASTEURELLA-PISCICIDA ISOLATED FROM CULTURED YELLOWTAIL IN JAPAN
DRUG-RESISTANCE AND BROAD GEOGRAPHICAL-DISTRIBUTION OF IDENTICAL R-PLASMIDS OF PASTEURELLA-PISCICIDA ISOLATED FROM CULTURED YELLOWTAIL IN JAPAN
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DOI：
10.1111/j.1348-0421.1993.tb03186.x
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发表时间：
1993-01-01
影响因子：
2.6
通讯作者：
AOKI, T
中科院分区：
文献类型：
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作者：
KIM, E;AOKI, T
12种化疗药物对175株杀鱼巴氏杆菌的最低抑菌浓度测定（Seriola quinqueradiata）的152株（87%）对氨苄青霉素（AP）、氯霉素（CP）、卡那霉素（KM）、萘啶酸（NA）、磺胺间甲氧嘧啶（SA）、四环素（TC）、和/或甲氧苄啶（TMP）。其余23株菌株对所有供试药物敏感：AP、头孢唑啉、CP、氟苯尼考（FF）、呋喃唑酮、KM、NA、新生霉素、SA、链霉素、TC和TMP。FF显示出对杀鱼假单胞菌最有效的抗菌活性，MIC范围为0.004至0.6 μ g/ml。152株耐药菌株中有149株携带编码Cp Km Sa Tc、Km Sa Tc、Km Sa和Sa耐药性之一的可转移R质粒。在杀鱼腥藻中鉴定的可转移R质粒的最常见的抗性标记是Km Sa Tc。R质粒编码三种不同的抗性标记是非常相似的基础上，他们的限制性内切酶消化模式。所选的9个可转移的R质粒DNA之间存在同源性。我们的研究结果表明，携带可转移的R质粒具有相同的DNA结构的多药耐药菌株的杀鱼假单胞菌在黄尾鱼养殖场是常见的，R质粒已被保留在杀鱼假单胞菌种群中，根据地理和年份，其DNA结构没有变化。
An MIC test of 12 chemotherapeutic agents performed on 175 strains of Pasteurella piscicida collected from cultured yellowtail (Seriola quinqueradiata) in different areas of Japan from 1989 to 1991 revealed 152 strains (87%) with resistance to combinations of ampicillin (AP), chloramphenicol (CP), kanamycin (KM), nalidixic acid (NA), sulfamonomethoxine (SA), tetracycline (TC), and/or trimethoprim (TMP). The remaining 23 strains were sensitive to all the drugs tested: AP, cefazolin, CP, florfenicol (FF), furazolidone, KM, NA, novobiocin, SA, streptomycin, TC, and TMP. FF showed the most effective antibacterial activity against P. piscicida with MICs ranging from 0.004 to 0.6 mug/ml. One hundred and forty-nine of the 152 resistant strains carried transferable R plasmids encoding one of the Cp Km Sa Tc, Km Sa Tc, Km Sa, and Sa resistance. The most common resistance marker of transferable R plasmids identified in P. piscicida was Km Sa Tc. R plasmids encoding three different resistant markers were very similar on the basis of their digestion patterns with restriction endonucleases. There was homology among the DNAs of nine transferable R plasmids selected. Our findings suggest that multiple drug resistant strains of P. piscicida carrying transferable R plasmids with the same DNA structure are common in yellowtail farms and that the R plasmid has been retained within the P. piscicida population without change in their DNA structure according to geography and year.