Actin homolog MreBH governs cell morphogenesis by localization of the cell wall hydrolase LytE
Actin homolog MreBH governs cell morphogenesis by localization of the cell wall hydrolase LytE
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DOI：
10.1016/j.devcel.2006.07.017
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发表时间：
2006-09-01
影响因子：
11.8
通讯作者：
Errington, Jeff
中科院分区：
文献类型：
--
作者：
Carballido-Lopez, Rut;Formstone, Alex;Errington, Jeff
MreB蛋白是细菌肌动蛋白的同源物；参与细胞形态发生和各种其他细胞过程。然而，MreB使用的效应蛋白在很大程度上仍不清楚。枯草芽孢杆菌有三种MreB亚型。MBL和可能的MreB先前已经被证明与细胞壁的合成有关。我们现在已经发现，在枯草杆菌中，第三个亚型MreBH与另外两个MreB亚型共存，并且在细胞形态发生中也有重要的作用。MreBH可以物理上与细胞壁水解酶Lyte相互作用，并且是其细胞外定位的螺旋模式所必需的。此外，Lyte和mreBH突变体表现出类似的细胞壁相关缺陷。我们认为杆状枯草杆菌的可控伸长依赖于MreB蛋白定义的螺旋束中细胞壁合成和水解的协调。我们的数据还表明，与细胞内肌动蛋白束的物理相互作用可以影响细胞外效应器的后期定位模式。
MreB proteins are bacterial actin homologs; involved in cell morphogenesis and various other cellular processes. However, the effector proteins used by MreBs remain largely unknown. Bacillus subtilis has three MreB isoforms. Mbl and possibly MreB have previously been shown to be implicated in cell wall synthesis. We have now found that the third isoform, MreBH, colocalizes with the two other MreB isoforms in B. subtilis and also has an important role in cell morphogenesis. MreBH can physically interact with a cell wall hydrolase, LytE, and is required for its helical pattern of extracellular localization. Moreover, lytE and mreBH mutants exhibit similar cell-wall-related defects. We propose that controlled elongation of rod-shaped B. subtilis depends on the coordination of cell wall synthesis and hydrolysis in helical tracts defined by MreB proteins. Our data also suggest that physical interactions with intracellular actin bundles can influence the later localization pattern of extracellular effectors.