A preliminary study on the genetic profile of cag pathogenicity-island and other virulent gene loci of Helicobacter pylori strains from Turkey
A preliminary study on the genetic profile of cag pathogenicity-island and other virulent gene loci of Helicobacter pylori strains from Turkey
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DOI：
10.1016/j.meegid.2007.03.002
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发表时间：
2007-07-01
影响因子：
3.2
通讯作者：
Ahmed, Niyaz
中科院分区：
文献类型：
--
作者：
Salih, Bank A.;Abaslyanik, M. Fatih;Ahmed, Niyaz
幽门螺杆菌遗传多样性影响与疾病结局相关的毒力因子的功能和抗原性。基因图谱分析cag致病岛（派）内外基因位点的分布。H.用PCR方法对35例患者（胃炎21例，消化性溃疡14例）的pylori菌株进行了分析。使用跨越3 '端、cagA、cagA的启动子区、cagE、cagT、5 '端（LEC）、极右端、可塑性区开放阅读框（ORF）、oipA（Hp 0638）和vacA等位基因的引物来评估cag派的谱。我们发现很少有完整的cag派的菌株检查。在胃炎和PUD菌株中，LEC 1（9.5%对14.3%）、LEC 2（4.8%对14.3%）、cagT（33.3%对28.6%）、cagE（28.6%对28.6%）和cagA启动子区（19.0%对42.9%）分别发现缺失。cagA基因在57例中检出。1%的胃炎和92.9%的PUD相关菌株。cagRJ区域也显示其许多基因的缺失。OipA（Hp 0638）基因在胃炎和PUD中的检出率分别为80.9%和92.9%。PUD菌株的塑性区ORF JHP 912和JHP 931占优势。PUD以vacA-s Ⅰ a-m Ⅰ a基因型为主，而Gastrifis以s（2）m（2）基因型为主。该综合分析显示cag PAL内外的几个基因缺失。然而，cagA、oipA、JHP 912、JHP 931和vacA-sla-mla在PUD菌株中比胃炎相关菌株更占优势，表明遗传多样性对疾病进展和临床结果的重要性。(c)2007 Elsevier B. V.保留所有权利。
Helicobacter pylori genetic diversity affects the function and antigenicity of virulence factors associated with the disease outcome. Gene profile was done to identify the distribution of gene loci within and outside the cag pathogenicity-island (PAI). H. pylori strains from 35 patients [21 gastritis, 14 peptic ulcer diseases (PUD)] were analyzed using PCR. The profile of the cag PAI was evaluated using primers spanning the 3 ' end, cagA, promoter region of the cagA, cagE, cagT, 5 ' end (LEC), extreme right end, plasticity region open reading frames (ORFs), oipA (Hp0638) and vacA alleles. We found few intact cag PAI in the strains examined. Deletions were found in LEC 1 (9.5% versus 14.3%), LEC2 (4.8% versus 14.3%), cagT (33.3%, versus 28.6%), cagE (28.6% versus 28.6%) and the promoter region of the cagA (19.0% versus 42.9%) of gastritis and PUD strains, respectively. The cagA gene was detectable in 57. 1 % of gastritis and 92.9% of PUD-associated strains. The cagRJ region also showed deletions for many of its genes. The oipA (Hp0638) gene was detected in 80.9% of gastritis and in 92.9% of PUD strains. The plasticity region ORFs JHP912 and JHP931 were predominant in PUD strains. The vacA-s I a-m I a genotype was predominant in PUD, while s(2)m(2) in gastrifis strains. This comprehensive analysis showed deletions in several genes within and outside the cag PAL However, cagA, oipA, JHP912, JHP931 and vacA-sla-mla were more predominant in PUD strains than gastritis-associated strains, suggesting the importance of genetic diversity on the disease progression and clinical outcome. (c) 2007 Elsevier B.V. All rights reserved.