Flexible and stretchable nanowire-coated fibers for optoelectronic probing of spinal cord circuits.
Flexible and stretchable nanowire-coated fibers for optoelectronic probing of spinal cord circuits.
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DOI：
10.1126/sciadv.1600955
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发表时间：
2017-03
期刊：
影响因子：
13.6
通讯作者：
Anikeeva P
中科院分区：
文献类型：
--
作者：
Lu C;Park S;Richner TJ;Derry A;Brown I;Hou C;Rao S;Kang J;Mortiz CT;Fink Y;Anikeeva P
在自由活动中，可伸展的光电纤维询问脊髓回路。对瘫痪脊髓损伤后导致功能丧失和恢复的神经通路的研究需要调节和记录特定神经元的电生理活动的设备。这些装置必须足够灵活，以匹配神经组织的低弹性模数，并承受正常运动期间脊髓经历的反复应变。我们报道了柔性的、可伸展的探针，它由热拉伸的聚合物纤维组成，表面覆盖着微米厚的银纳米线导电网状物。这些混合探针在可见光范围内保持较低的光传输损耗，并在超过哺乳动物脊髓中出现的应变下保持适合细胞外记录的阻抗。在自由活动的小鼠身上的评估证实了这些探针记录脊髓内源性电生理活动的能力。在表达通道视紫红质2的转基因小鼠中，显示出同时的刺激和记录，其中光激发引起肌电活动和后肢运动与脊髓中测量的局部场电位相关。
Stretchable optoelectronic fibers interrogate spinal cord circuits during free behavior. Studies of neural pathways that contribute to loss and recovery of function following paralyzing spinal cord injury require devices for modulating and recording electrophysiological activity in specific neurons. These devices must be sufficiently flexible to match the low elastic modulus of neural tissue and to withstand repeated strains experienced by the spinal cord during normal movement. We report flexible, stretchable probes consisting of thermally drawn polymer fibers coated with micrometer-thick conductive meshes of silver nanowires. These hybrid probes maintain low optical transmission losses in the visible range and impedance suitable for extracellular recording under strains exceeding those occurring in mammalian spinal cords. Evaluation in freely moving mice confirms the ability of these probes to record endogenous electrophysiological activity in the spinal cord. Simultaneous stimulation and recording is demonstrated in transgenic mice expressing channelrhodopsin 2, where optical excitation evokes electromyographic activity and hindlimb movement correlated to local field potentials measured in the spinal cord.