Mechanical characterization of yeast cells: effects of growth conditions
Mechanical characterization of yeast cells: effects of growth conditions
复制标题
DOI：
10.1111/lam.12468
复制
发表时间：
2015-10
影响因子：
2.4
通讯作者：
Achim Overbeck;I. Kampen;A. Kwade
中科院分区：
文献类型：
--
作者：
Achim Overbeck;I. Kampen;A. Kwade
工业生物技术利用微生物细胞生产各种产品。虽然所讨论的生物体在其遗传和分子特性方面得到了很好的理解，但对其机械特性知之甚少。以前的工作已经建立了一个测试程序，使用配备有平冲探针的纳米压痕仪，允许两个平行表面之间的压缩，用于单个酿酒酵母细胞。所得的力-位移曲线清楚地显示了细胞的破裂，并用于确定破裂力、破裂能量和相对变形等特征值。本研究探讨了机械特性的酵母细胞的影响下，不同的培养参数，即pH值，温度，通气量，搅拌速度和培养基成分。据观察，只有温度和培养基组成显示出对细胞的机械性能的显着影响。修炼的时候，温度越高，爆发力和能量就越低。对数据的进一步分析表明，细胞的机械特性仅受也对生长速率有影响的参数的影响。总之，较高的生长速率导致酵母细胞的机械强度较低。
Industrial biotechnology uses microbiological cells to produce a wide range of products. While the organisms in question are well understood regarding their genetic and molecular properties, less is known about their mechanical properties. Previous work has established a testing procedure for single Saccharomyces cerevisiae cells using a Nanoindenter equipped with a Flat Punch probe, allowing the compression between two parallel surfaces. The resulting force‐displacement curves clearly showed the bursting of the cells and served to determine characteristic values such as the bursting force, bursting energy and relative deformation. This study examined the mechanical characteristics of yeast cells under the influence of varying cultivation parameters, namely the pH value, temperature, aeration rate, stirrer speed and culture medium composition. It was observed that only temperature and medium composition showed significant effect on the mechanical properties of the cells. Higher temperatures during cultivation caused lower bursting forces and energies. Further analysis of the data showed that the mechanical characteristics of the cells were only influenced by parameters which also had an influence on the growth rate. In conclusion, higher growth rates result in a lower mechanical strength of the yeast cells.