Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440
Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440
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DOI：
10.1046/j.1462-2920.2002.00366.x
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发表时间：
2002-12-01
影响因子：
5.1
通讯作者：
Fraser, CM
中科院分区：
文献类型：
--
作者：
Nelson, KE;Weinel, C;Fraser, CM
恶臭假单胞菌是一种代谢多样的腐生土壤细菌，已被证明是克隆外源基因的生物安全宿主。这种细菌在生物技术应用方面也具有相当大的潜力。菌株KT2440的6.18 Mb基因组序列分析揭示了其多种运输和代谢系统。尽管致病性铜绿假单胞菌具有高度的基因组保守性（85%的预测编码区是共享的），但缺乏包括外毒素a和III型分泌系统在内的关键毒力因子。基因组分析揭示了恶臭杆菌的非致病性，并指出了其在农业、生物催化、生物修复和生物塑料生产方面的潜在新应用。
Pseudomonas putida is a metabolically versatile saprophytic soil bacterium that has been certified as a biosafety host for the cloning of foreign genes. The bacterium also has considerable potential for biotechnological applications. Sequence analysis of the 6.18 Mb genome of strain KT2440 reveals diverse transport and metabolic systems. Although there is a high level of genome conservation with the pathogenic Pseudomonad Pseudomonas aeruginosa (85% of the predicted coding regions are shared), key virulence factors including exotoxin A and type III secretion systems are absent. Analysis of the genome gives insight into the non-pathogenic nature of P. putida and points to potential new applications in agriculture, biocatalysis, bioremediation and bioplastic production.