The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases
The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases
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DOI：
10.1111/j.1742-4658.2011.08033.x
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发表时间：
2011-04-01
期刊：
影响因子：
5.4
通讯作者：
Hirt, Heribert
中科院分区：
文献类型：
--
作者：
Forzani, Celine;Carreri, Alessandro;Hirt, Heribert
在拟南芥中，丝氨酸/苏氨酸蛋白激酶氧化信号诱导 1 (OXI1) 介导氧化应激信号传导。它的活性是丝裂原激活蛋白激酶 (MAP-Ks)、MPK3 和 MPK6 响应氧化应激的完全激活所必需的。此外，丝氨酸/苏氨酸蛋白激酶 Pto 相互作用 l-2 (PTI1-2) 已位于 OXI1 的下游，但 PTI1-2 是否通过 MAPK 级联传递信号尚不清楚。使用酵母双杂交筛选，我们表明 OXI1 也与 PTI1-4 相互作用，因为 OXI1 和 PTI1-4 是应激反应基因，并且在相同组织中表达。因此，进行了研究以确定PTI1-4是否位于OXI1/MAPK信号通路中。通过体内免疫共沉淀实验证实了 OXI1 和 PTI1-4 之间的相互作用。 OXI1和PTI1-4是MPK3和MPK6的体外底物。尽管未检测到 OXI1 与 MPK3 或 MPK6 之间存在直接相互作用，但体外结合研究显示 MPK3 或 MPK6 与 PTI1-4 之间存在相互作用。此外，PTI1-4和MPK6在体内被发现处于同一蛋白质复合物中。这些结果表明 PTI1-4 通过 OXI1 和 MPK6 信号级联传递信号。
In Arabidopsis thaliana, the serine/threonine protein kinase oxidative signal-inducible 1 (OXI1), mediates oxidative stress signalling. Its activity is required for full activation of the mitogen-activated protein kinases (MAP-Ks), MPK3 and MPK6 in response to oxidative stress, In addition, the serine/threonine protein kinase Pto-interacting l-2 (PTI1-2) has been positioned downstream from OXI1, but whether PTI1-2 signals through MAPK cascades is unclear. Using a yeast two-hybrid screen we show that OXI1 also interacts with PTI1-4 is OXI1 and PTI1-4 are stress-responsive genes and are expressed in the same tissues. Therefore, studies were undertaken to determine whether PTI1-4 is positioned in the OXI1/MAPK signalling pathway. The interaction between OXI1 and PTI1-4 was confirmed by using in vivo co-immunoprecipitation experiments. OXI1 and PTI1-4 were substrates of MPK3 and MPK6 in vitro. Although no direct interaction was detected between OXI1 and MPK3 or MPK6, in vitro binding studies showed interactions between MPK3 or MPK6 with PTI1-4. In addition, PTI1-4 and MPK6 were found in vivo in the same protein complex. These results demonstrate that PTI1-4 signals via OXI1 and MPK6 signalling cascades.