FRET-based biosensors for protein kinases: illuminating the kinome
FRET-based biosensors for protein kinases: illuminating the kinome
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DOI：
10.1039/b706628g
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发表时间：
2007-01-01
影响因子：
--
通讯作者：
Allen, Michael D.
中科院分区：
文献类型：
--
作者：
Zhang, Jin;Allen, Michael D.
蛋白激酶是细胞内信号传导途径的重要组成部分，其通过下游靶标的磷酸化来传递信号，从而改变其功能。有效的信号转导需要在特定的生物学背景下进行精确的激酶调节，这使得原位研究其动力学的工具对于理解激酶功能至关重要。在这篇文章中强调的是遗传编码，FRET为基础的激酶生物传感器的设计，其实施的例子，以研究激酶调节在活的生物背景下，具有高的空间和时间分辨率。
Protein kinases are crucial components of intracellular signaling pathways which transmit signals by phosphorylation of downstream targets, altering their function. Efficient signal transduction requires precise kinase regulation within specific biological contexts, making tools that allow study of their dynamics in situ critical for understanding kinase function. Highlighted in this article is the design of genetically-encodable, FRET-based kinase biosensors with examples of their implementation to study kinase regulation in live biological contexts with high spatial and temporal resolution.