Application of a bacterial whole cell biosensor for the rapid detection of cytotoxicity in heavy metal contaminated seawater
Application of a bacterial whole cell biosensor for the rapid detection of cytotoxicity in heavy metal contaminated seawater
复制标题
细菌全细胞生物传感器在重金属污染海水细胞毒性快速检测中的应用
DOI：
10.1016/j.chemosphere.2018.02.097
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发表时间：
2018-06-01
期刊：
影响因子：
8.8
通讯作者：
Huang, Wei E.
中科院分区：
文献类型：
--
作者：
Cui, Zhisong;Luan, Xiao;Huang, Wei E.
以宿主菌A.将pWH1274_lux质粒导入baylyi ADP 1中，并将其应用于重金属污染海水的细胞毒性检测。基因盒luxCDABE由pWH1274_lux上组成型表达的启动子P-ret控制，并且生物传感器的生物发光强度与毒性化合物的浓度成比例地降低。A. baylyi Tox 2对盐度有较强的耐受性，适用于海水样品。A.在不同浓度的人工海水中，对贝氏鳅毒素2（BaylyiTox 2）和丘拉幕府鳅（Mugilogobiuschulae）进行了重金属（Hg 2+、Zn 2+、Cu 2+、Cd 2+）暴露试验。baylyi Tox 2毒力测定及鱼体（M. Chulae）暴露试验。这表明，A. Baylyi Tox 2在重金属污染海水的细胞毒性检测方面与常规鱼类毒性试验相当。此外，A.使用Baylyi Tox 2评价现场采集的海水样品的细胞毒性。结果表明，A.通过ICP-MS检测样品中的Baylyi Tox 2和重金属浓度。两个海水样品，其中含有高浓度的总重金属，表现出更强的细胞毒性比样品含有低浓度的重金属。总之，A. Baylyi Tox 2可作为水生动物的替代工具用于评价海洋环境中重金属污染的细胞毒性。(C)2018爱思唯尔有限公司版权所有。
toxicity biosensor Acinetobacter baylyi Tox2 was constructed with the host strain A. baylyi ADP1 harboring a new and medium-copy-number plasmid pWH1274_lux, and was applied to detect the cytotoxicity of heavy metal contaminated seawater. The gene cassette luxCDABE was controlled by constitutively expressed promoter P-ret on pWH1274_lux and the bioluminescence intensity of the biosensor reduces in proportional to the concentrations of toxic compounds. A. baylyi Tox2 exhibits tolerance to salinity, hence it is applicable to seawater samples. A. baylyi Tox2 and Mugilogobius chulae were exposed to different concentrations of heavy metals (Hg2+, Zn2+, Cu2+, and Cd2+) in artificial seawater for performance comparison and Pearson correlation analysis showed a significant correlation (p < 0.01) between A. baylyi Tox2 toxicity detection and the fish (M. chulae) exposure test. This suggests that the performance of A. baylyi Tox2 is comparable to the conventional fish toxicity test in terms of cytotoxicity detection of heavy metal contaminated seawater. Furthermore, A. baylyi Tox2 was used to evaluate cytotoxicity of field-collected seawater samples. The results indicate that there was a significant correlation between the luminescence inhibition ratio (IR) of A. baylyi Tox2 and heavy metal concentrations detected by ICP-MS in the samples. Two seawater samples, which contained a high concentration of total heavy metals, exhibited stronger cytotoxicity than the samples containing low concentrations of heavy metals. In conclusion, A. baylyi Tox2 can be used as an alternative tool to aquatic animals for the evaluation of the cytotoxicity of heavy metal contamination in the marine environment. (C) 2018 Elsevier Ltd. All rights reserved.