Invasive properties of fibroblast-like synoviocytes: correlation with growth characteristics and expression of MMP-1, MMP-3, and MMP-10
Invasive properties of fibroblast-like synoviocytes: correlation with growth characteristics and expression of MMP-1, MMP-3, and MMP-10
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DOI：
10.1136/ard.61.11.975
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发表时间：
2002-11-01
影响因子：
27.4
通讯作者：
Huizinga, TWJ
中科院分区：
文献类型：
--
作者：
Tolboom, TCA;Pieterman, E;Huizinga, TWJ
背景：基质金属蛋白酶（MMPs）在类风湿性关节炎（RA）软骨破坏中起关键作用，该过程是由成纤维细胞样滑膜细胞（FLS）介导的。目的：探讨关节炎性关节炎滑膜细胞体外侵袭性与基质金属蛋白酶组织抑制剂TIMP-1、TIMP-2、MMP 1- 14,17,19、组织蛋白酶- k表达的关系。方法：从56例RA患者（30例RA， 17例OA， 9例AVN）中提取FLS。在transwell系统中测量人工基质（Matrigel）中FLS的侵袭性生长。计数通过基质迁移的细胞数量。培养7天后，通过计数FLS来测定增殖率。通过逆转录聚合链反应研究MMPs、cathepsin-K和TIMPs的表达，这些表达与一个家庭基因- β -actin的表达有关。结果：RA的FLS比OA和AVN的FLS具有更大的侵袭性生长。RA通过基质膜生长的细胞中位数为4788个，明显高于OA的1875个(p
Background: Matrix metalloproteinases (MMPs) have a pivotal role in the destruction of cartilage in rheumatoid arthritis (RA), which is mediated by the fibroblast-like synoviocytes (FLS).Objective: To examine the in vitro invasiveness of synoviocytes obtained from inflamed joints of patients with arthritis in relation to the expression of MMP 1-14, 17, 19, cathepsin-K, the tissue-inhibitors of matrix metalloproteinases TIMP-1 and TIMP-2 by FLS.Methods: FLS were derived from 56 patients (30 with RA, 17 with osteoarthritis (OA), and nine with avascular necrosis (AVN)). Invasive growth of FLS through an artificial matrix (Matrigel) was measured in a transwell system. The number of cells that migrated through the matrix were counted. Proliferation rate was determined by counting the FLS after seven days of culturing. Expression of MMPs, cathepsin-K and TIMPs was investigated with reverse transcriptase-polymerise chain reaction and related to the expression of a household gene, beta-actin.Results: FLS from RA showed greater invasive growth than FLS from OA and AVN. The median number of cells that grew through the matrix membrane was 4788 for RA, significantly higher than the number for OA, 1875 (p