Expression profiling using human tissues in combination with RNA amplification and microarray analysis: assessment of Langerhans cell histiocytosis
Expression profiling using human tissues in combination with RNA amplification and microarray analysis: assessment of Langerhans cell histiocytosis
复制标题
使用人体组织结合 RNA 扩增和微阵列分析进行表达谱分析：朗格汉斯细胞组织细胞增多症的评估
作者：
K. McClain;Y.;J. Hicks;Leif E. Peterson;X. Yan;S. Che;S. Ginsberg
分子遗传学的进步导致了人类基因组的测序，并且表达数据变得可用于整个身体的许多不同组织，允许对诸如发育、分化、稳态以及最终的疾病发病机制等关键概念进行令人兴奋的假设检验。目前，评估基因表达的最佳方法是评估单个细胞，无论是在活的制剂中生理学鉴定，还是在体外或体内固定细胞中通过免疫细胞化学或组织化学程序鉴定。不幸的是，从单个细胞收获的RNA的量对于标准RNA提取方法是不够的。因此，基于指数聚合酶链式反应（PCR）的分析，和线性RNA扩增，包括扩增的反义（aRNA）RNA扩增和新开发的末端连续（TC）RNA扩增方法，已经与显微切割程序如激光捕获显微切割（LCM）结合使用，为了使微阵列平台能够在从多种人类组织来源获得的单个细胞群中使用，例如活组织检查衍生的样品，包括朗格汉斯细胞组织细胞增多症（LCH））以及死后脑样品用于高通量表达谱分析和相关下游遗传分析。
Summary.Advances in molecular genetics have led to sequencing of the human genome, and expression data is becoming available for many diverse tissues throughout the body, allowing for exciting hypothesis testing of critical concepts such as development, differentiation, homeostasis, and ultimately, disease pathogenesis. At present, an optimal methodology to assess gene expression is to evaluate single cells, either identified physiologically in living preparations, or by immunocytochemical or histochemical procedures in fixed cells in vitro or in vivo. Unfortunately, the quantity of RNA harvested from a single cell is not sufficient for standard RNA extraction methods. Therefore, exponential polymerase-chain reaction (PCR) based analyses, and linear RNA amplification including amplified antisense (aRNA) RNA amplification and a newly developed terminal continuation (TC) RNA amplification methodology have been used in combination with microdissection procedures such as laser capture microdissection (LCM) to enable the use of microarray platforms within individual populations of cells obtained from a variety of human tissue sources such as biopsy-derived samples {including Langerhans cell histiocytosis (LCH)} as well as postmortem brain samples for high throughput expression profiling and related downstream genetic analyses.
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影响因子：
--
作者：
Hemby, SE;Ginsberg, SD;Eberwine, JH
通讯作者：
Eberwine, JH
DOI：
10.1073/pnas.89.7.3010
发表时间：
1992-04-01
影响因子：
11.1
作者：
EBERWINE, J;YEH, H;COLEMAN, P
通讯作者：
COLEMAN, P
影响因子：
7
作者：
Heyer, LJ;Kruglyak, S;Yooseph, S
通讯作者：
Yooseph, S
乙醇和多聚甲醛对 RNA 产量和质量的影响。
影响因子：
2.7
作者：
Klimecki,WT;Futscher,BW;Dalton,WS
通讯作者：
Dalton,WS
DOI：
10.1073/pnas.87.5.1663
发表时间：
1990-03-01
影响因子：
11.1
作者：
VANGELDER, RN;VONZASTROW, ME;EBERWINE, JH
通讯作者：
EBERWINE, JH