Physical and functional interactions among basic chromosome organizational features govern early steps of meiotic chiasma formation
Physical and functional interactions among basic chromosome organizational features govern early steps of meiotic chiasma formation
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DOI：
10.1016/s0092-8674(02)01167-4
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发表时间：
2002-12-13
期刊：
影响因子：
64.5
通讯作者：
Kleckner, N
中科院分区：
文献类型：
--
作者：
Blat, Y;Protacio, RU;Kleckner, N
通过功能基因组学方法分析减数分裂重组揭示了不同组织决定因素之间突出的空间和功能相互作用。染色质环序列之间发生重叠;然而，这些序列通过它们的重组体在空间上被束缚到潜在的染色体轴上。减数分裂染色体蛋白，Red 1，定位于染色体轴，然而，Red 1加载调制的R/G-带isochores，从而散装染色质状态。重组也受到等容决定簇的调节：R带差异地有利于双链断裂（DSB）的形成，但不利于随后加载减数分裂RecA同源物Dmc 1。Red 1促进DSB在R带和G带中的形成，然后促进Dmc 1加载，特别是抵消不利的R带效应。这些复杂性的背景下，交叉形成的DNA和染色体轴水平上的一系列协调的局部变化进行了讨论。
Analysis of meiotic recombination by functional genomic approaches reveals prominent spatial and functional interactions among diverse organizational determinants. Recombination occurs between chromatin loop sequences; however, these sequences are spatially tethered to underlying chromosome axes via their recombinosomes. Meiotic chromosomal protein, Red1, localizes to chromosome axes; however, Red1 loading is modulated by R/G-bands isochores and thus by bulk chromatin state. Recombination is also modulated by isochore determinants: R-bands differentially favor double-strand break (DSB) formation but disfavor subsequent loading of meiotic RecA homolog, Dmc1. Red1 promotes DSB formation in both R- and G-bands and then promotes Dmc1 loading, specifically counteracting disfavoring R-band effects. These complexities are discussed in the context of chiasma formation as a series of coordinated local changes at the DNA and chromosome-axis levels.