Folate conjugated trimethyl chitosan/graphene oxide nanocomplexes as potential carriers for drug and gene delivery
Folate conjugated trimethyl chitosan/graphene oxide nanocomplexes as potential carriers for drug and gene delivery
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DOI：
10.1016/j.matlet.2014.03.133
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发表时间：
2014-06
影响因子：
3
通讯作者：
Hui-fang Hu;Cui Tang;C. Yin
中科院分区：
文献类型：
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作者：
Hui-fang Hu;Cui Tang;C. Yin
通过静电自组装制备叶酸偶联的三甲基壳聚糖（FTMC）/氧化石墨烯（GO）纳米复合物（FG NC），作为阿霉素（DOX）和质粒DNA（pDNA）的靶向递送载体。FG NC的直径为112.0 nm，厚度为3.038 nm。在Hela和A549细胞中观察到FG NC的细胞毒性可忽略不计。在具有叶酸受体的Hela细胞中较高的摄取水平证明了FG NCs的靶向能力。DOX和pDNA均能负载到FG NCs中，其中DOX的负载量达到30.9%，并且pDNA的迁移可以被完全抑制。因此，FG NCs可以作为抗癌药物和基因靶向递送的有希望的候选者。
Folate conjugated trimethyl chitosan (FTMC)/graphene oxide (GO) nanocomplexes (FG NCs) prepared via electrostatic self-assembly were here developed as a targeted delivery vehicle for both doxorubicin (DOX) and plasmid DNA (pDNA). FG NCs exhibited the diameter of 112.0 nm and the thickness of 3.038 nm. Negligible cytotoxicity of FG NCs was observed in Hela and A549 cells. The higher uptake level in Hela cells with folate-receptor proved the targeting ability of FG NCs. Both DOX and pDNA could be loaded into FG NCs, wherein the loading capacity of DOX reached 30.9% and migration of pDNA could be completely retarded. Therefore, FG NCs could be served as a promising candidate for targeted delivery of both anticancer drugs and genes.