Enhanced blood coagulation and antibacterial activities of carboxymethyl-kappa-carrageenan-containing nanofibers
Enhanced blood coagulation and antibacterial activities of carboxymethyl-kappa-carrageenan-containing nanofibers
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DOI：
10.1016/j.carbpol.2021.118541
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发表时间：
2021-08-14
影响因子：
11.2
通讯作者：
Kipper, Matt J.
中科院分区：
文献类型：
--
作者：
Madruga, Liszt Y. C.;Popat, Ketul C.;Kipper, Matt J.
理想的伤口敷料应具有生物相容性，抗菌活性高，促进血液凝固。为了赋予这些必要的功能，将羧甲基-卡帕-卡拉胶掺入聚乙烯醇纳米纤维（PVA-CMKC）中。对纳米纤维的抗菌活性进行了评价。两种重要的血液蛋白，纤维蛋白原和白蛋白的吸附也被评估。评估了血小板的粘附和活化以及全血的凝血能力，以表征纳米纤维促进止血的能力。用荧光显微镜和扫描电镜观察金黄色葡萄球菌和铜绿假单胞菌的粘附性和形态。与PVA纳米纤维相比，含有cmkc的纳米纤维在血小板粘附和活化、全血凝血试验中凝血率和纤维蛋白原吸附方面均有显著提高，显示出凝血活性。加入CMKC还可在孵育24小时后降低金黄色葡萄球菌和铜绿假单胞菌的粘附力和生存能力。PVACMKC纳米纤维在伤口愈合敷料方面具有潜在的应用前景。
Ideal wound dressings should be biocompatible, exhibit high antibacterial activity, and promote blood coagulation. To impart these imperative functions, carboxymethyl-kappa-carrageenan was incorporated into poly (vinyl alcohol) nanofibers (PVA-CMKC). The antibacterial activity of the nanofibers was evaluated. Adsorption of two important blood proteins, fibrinogen and albumin, was also assessed. The adhesion and activation of platelets, and the clotting of whole blood were evaluated to characterize the ability of the nanofibers to promote hemostasis. Adhesion and morphology of both Staphylococcus aureus and Pseudomonas aeruginosa were evaluated using fluorescence microscopy and scanning electron microscopy. CMKC-containing nanofibers demonstrated significant increases in platelet adhesion and activation, percentage of coagulation in whole blood clotting test and fibrinogen adsorption, compared to PVA nanofibers, showing blood coagulation activity. Incorporating CMKC also reduces adhesion and viability of S. aureus and P. aeruginosa bacteria after 24 h of incubation. PVACMKC nanofibers show potential application as dressings for wound healing applications.