Decreased expression of endometrial vessel AQP1 and endometrial epithelium AQP2 related to anovulatory uterine bleeding in premenopausal women
Decreased expression of endometrial vessel AQP1 and endometrial epithelium AQP2 related to anovulatory uterine bleeding in premenopausal women
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绝经前女性子宫内膜血管AQP1和子宫内膜上皮AQP2表达降低与无排卵子宫出血相关
DOI：
10.1097/gme.0b013e31816086ef
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发表时间：
2008-07-01
影响因子：
2.7
通讯作者：
Huang, He-Feng
中科院分区：
文献类型：
--
作者：
Feng, Chun;Sun, Chao-Chao;Huang, He-Feng
目的：水通道蛋白（Aquaporins，AQP）作为卵巢激素与子宫内膜周期性变化之间的介导者，可能参与了异常子宫出血的发生。本研究旨在探讨无排卵性子宫出血患者子宫内膜水通道蛋白（AQP）的表达特点及其与卵巢激素的关系。研究设计：61例绝经前无排卵性子宫出血患者和108例正常月经周期患者纳入本研究。对无排卵性子宫出血患者和正常对照妇女进行子宫内膜活检。血清雌二醇和孕酮浓度测定与酶联免疫吸附试验的同一天，子宫内膜活检进行。采用逆转录聚合酶链反应检测AQP 1和AQP 2 mRNA的表达。采用免疫组织化学方法对子宫内膜中AQP 1和AQP 2进行定位，并通过图像分析/测量系统对它们的表达进行定量。结果：AQP 1定位于小血管内皮，AQP 2主要定位于管腔上皮和腺上皮。在正常子宫内膜中，AQP 1和AQP 2 mRNA和蛋白的表达水平在分泌期高于增殖期（P < 0.01），且其表达水平与血清类固醇激素水平有关（P < 0.01）。AQP 1和AQP 2在无排卵性子宫出血患者子宫内膜中的表达均低于正常子宫内膜（P < 0.01）。无排卵性子宫出血组AQP表达与卵巢激素水平无相关性（P > 0.05）。结论：子宫内膜AQP 1/AQP 2的周期性表达与激素水平相关，可能是维持正常子宫内膜功能的重要因素，子宫内膜血管或上皮AQP 1/AQP 2表达降低可能与无排卵性子宫出血的发生有关。
Objective:Aquaporins (AQPs) may be involved in the occurrence of abnormal uterine bleeding as the mediators between ovarian steroids and cyclic endometrial changes. The aim of the present study was to investigate the characteristics of endometrial AQPs in women with anovulatory uterine bleeding and explore the relationship between endometrial AQPs and ovarian steroids. Design:Sixty-one women with premenopausal anovulatory uterine bleeding and 108 women with normal cycles were involved in this study. Endometrial biopsies were obtained from the women with anovulatory uterine bleeding and normal control women. Serum estradiol and progesterone concentrations were measured with enzyme-linked immunosorbent assay on the same day as an endometrial biopsy was performed. AQP1 and AQP2 mRNA expression was evaluated using reverse-transcriptase polymerase chain reaction. Immunohistochemistry was used to localize AQP1 and AQP2 in the endometrium, and their expression was quantified by an image analysis/measuring system. Results:AQP1 was located in the endothelium of small vessels, whereas AQP2 was mainly found in luminal and glandular epithelium. The expression levels of AQP1 and AQP2 mRNA and protein were higher in the secretory phase than those in the proliferative phase (P < 0.01) in normal endometrium, and their expression was related to serum steroid hormones (P < 0.01). However, the expression of AQP1 and AQP2 decreased in the endometrium in anovulatory uterine bleeding comparing with normal endometrium (P < 0.01). The correlation between AQP expression and ovarian steroids vanished (P > 0.05) in anovulatory uterine bleeding. Conclusions:Our findings indicate that cyclic expression of endometrial AQP1/AQP2 correlated with steroid hormone levels may be essential to normal endometrial function and decreased AQP1/AQP2 expression in endometrial vessels or epithelium may be involved in the occurrence of anovulatory uterine bleeding.