Comparison of rapid and simple colorimetric microplate assays as an index of bacterial count
Comparison of rapid and simple colorimetric microplate assays as an index of bacterial count
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DOI：
10.1016/s0956-7135(03)00116-6
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发表时间：
2004-09-01
期刊：
影响因子：
6
通讯作者：
Yano, T
中科院分区：
文献类型：
--
作者：
Kuda, T;Shimizu, K;Yano, T
我们研究了快速和简单的比色微孔板测定法与胰蛋白胨酵母葡萄糖肉汤（TYG）含有氧化还原（氧化还原）指示剂（alamarBlue，NITS和WST-8），AquaTest（大肠菌群的选择性培养基）和PYP肉汤含有pH指示剂（酚红），以评估使用大肠杆菌，金黄色葡萄球菌和蜡样芽孢杆菌菌株作为测试生物体的细胞计数。在所有氧化还原系统中，通过在35 ℃下孵育数小时来估计需氧细胞浓度是可能的。虽然MTS或WST的光密度高于alamarBlue，但获得结果的时间（可能与倍增时间相关）更长。AquaTest中的颜色变化比含有alamarBlue和PYP的TYG晚200-300分钟。(C)2003爱思唯尔有限公司。保留所有权利。
We investigated rapid and simple colorimetric microplate assays with tryptone-yeast-glucose broth (TYG) containing oxidation-reduction (redox) indicators (alamarBlue, NITS and WST-8), AquaTest (selective medium for coliforms) and PYP broth containing pH indicator (phenol-red) to assess cell counts using Escherichia coli, Staphylococcus aureus and Bacillus cereus strains as test organisms. In all redox systems, the estimation of aerobic cell concentration was possible by incubating at 35 degreesC for several hours. Although optical densities for MTS or WST were higher than that of alamarBlue, the time to obtain results (which may be correlated with doubling time) was longer. Color changes in AquaTest were 200-300 min later than for TYG containing alamarBlue and PYP. (C) 2003 Elsevier Ltd. All rights reserved.