Sca-1 and Thy-1 accelerate neuron-like differentiation in bone marrow stromal cells.
Sca-1 and Thy-1 accelerate neuron-like differentiation in bone marrow stromal cells.
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发表时间：
2006
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通讯作者：
Takashi Mizobe;K. Kidoguchi;M. Tamaki;T. Sasayama;T. Kondoh;E. Kohmura
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作者：
Takashi Mizobe;K. Kidoguchi;M. Tamaki;T. Sasayama;T. Kondoh;E. Kohmura
用磁珠对EGFP转基因小鼠骨髓间质细胞进行分类，磁珠表面标记为Sca-1和Thy-1。然后将细胞在器官型海马片上或与神经元细胞饲养器在培养皿中共培养。在海马区，Sca-1和Thy-1阳性细胞呈现神经元样形态的电位均比阴性细胞高4 ~ 8倍。在培养皿中，阴性细胞很少存活，阳性细胞均存活，并呈神经元样分化。在两种培养条件下，维甲酸的补充均能促进分化。分化后的Sca-1和Thy-1阳性细胞免疫组化GFAP和neun阴性，巢蛋白、神经丝蛋白和nse阳性。骨髓细胞的神经元样分化可以通过选择细胞表面蛋白来增强。
Bone marrow stromal cells taken from EGFP transgenic mice were sorted by magnetic beads with surface markers for Sca-1 and Thy-1. The cells were then co-cultured on organotypic hippocampal slice or with neuronal cell feeder in dish. On hippocampus, both Sca-1 and Thy-1 positive cells showed 4- 8 folds higher potential to show neuron-like morphology than negative cells. In dish, negative cells fewly survived but each positive cells survived and showed neuron-like differentiation. In both culture condition, retinoic acid supplement accelerate differentiation. Differentiated Sca-1 and Thy-1 positive cells were immunohistochemically GFAP- and NeuN-negative but nestin-, neurofilament- and NSE-positive. Neuron-like differentiation of bone marrow cells can be enhanced by selection using cell surface proteins.