DISSIMILATORY NITRATE UPTAKE IN PARACOCCUS-DENITRIFICANS VIA A DELTA-MU-H+-DEPENDENT SYSTEM AND A NITRATE-NITRITE ANTIPORT SYSTEM
DISSIMILATORY NITRATE UPTAKE IN PARACOCCUS-DENITRIFICANS VIA A DELTA-MU-H+-DEPENDENT SYSTEM AND A NITRATE-NITRITE ANTIPORT SYSTEM
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DOI：
10.1016/0005-2728(83)90049-x
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
STOUTHAMER, AH
中科院分区：
文献类型：
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作者：
BOOGERD, FC;VANVERSEVELD, HW;STOUTHAMER, AH
采用氧化剂脉冲法研究了在O2、NO3-、NO2-和N2 O还原过程中，厌氧发酵P. acetificans细胞氧化H2时呼吸驱动的质子转运。厌氧电子传递和相关的质子易位的简化方案表明，与测量的矢量。H+/氧化剂的比例是一致的。研究了铜绿假单胞菌全细胞吸收NO3-的动力学和能量学。H2的消耗或N2 O的生产后，除了NO3-的细胞悬浮液，这间接给出了有关NO3-的吸收（和减少）的信息进行了测量。H2消耗或N2 O的生产出现滞后相时，通过添加硫氰酸盐，羰基氰间氯苯腙或三苯基甲基溴化鏻的膜电位消散。当NO2-存在时，在引入NO3-的时刻没有观察到这些滞后期。根据这些发现，可以得出结论，有2个吸收系统的NO3-。一个系统是依赖于质子动力，并可能用于启动NO3-吸收。另一个是NO3-/NO2-反向转运体，其功能是从第一个系统接管NO3-摄取。
Respiration-driven proton translocation was studied with the oxidant pulse method for cells of denitrifying P. denitrificans oxidizing H2 during reduction of O2, NO3-, NO2- or N2O. A simplified scheme of anaerobic electron transport and associated proton translocations shown that is consistent with the measured .vector.H+/oxidant ratios. The kinetics and energetics of NO3- uptake in whole cells of P. denitrificans were studied. H2 consumption or N2O production was measured after addition to NO3- to a cell suspension, which indirectly gave information about uptake (and reduction) of NO3-. A lag phase in H2 consumption or N2O production appeared whenever the membrane potential was dissipated by addition of thiocyanate, carbonyl cyanide m-chlorophenylhydrazone or triphenylmethylphosphonium bromide. These lag phases were not observed when NO2- was present at the moment of introduction of NO3-. On the basis of these findings it is concluded that there are 2 uptake systems for NO3-. One system is dependent on the protonmotive force and is probably used for initiation of NO3- uptake. The other is an NO3-/NO2- antiport and its function is to take over NO3- uptake from the first system.