NMR Experiments for the Analysis of Mixtures: Beyond 1D 1H Spectra
NMR Experiments for the Analysis of Mixtures: Beyond 1D 1H Spectra
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DOI：
10.2174/138620712798280853
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发表时间：
2012-01-01
影响因子：
1.8
通讯作者：
Furrer, Julien
中科院分区：
文献类型：
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作者：
Jeannerat, Damien;Furrer, Julien
核磁共振波谱中最先进的技术和方法通过更好地选择核磁共振脉冲序列和采集参数，使得在比经典方法更少的实验时间内获得信息非常丰富的高质量谱成为可能。本文介绍了一些最新的核磁共振方法，可以快速鉴定、指认和表征混合物中的组分。简要讨论了不同核磁共振脉冲序列的相对优点，并对快速获得无伪影数据的首选序列提出了建议。本文综述了扩散实验(DOSY)、HSQC和HMBC实验、利用混叠特性的超分辨2D光谱以及NOESY/ROESY实验。将特别表明，与相应的2D序列相比，选择性1D NOESY/ROESY序列可以提供更多的信息，并在更少的实验时间内达到更高的分辨率。
State-of-the-art technologies and methodologies in NMR spectroscopy make it possible to obtain very informative and high-quality spectra in much less experimental time than classical methods by making better choices of NMR pulse sequences and acquisition parameters. This review presents some recent NMR methods allowing rapid identification, assignment and structural characterization of the components in mixtures. The relative merits of the different NMR pulse sequences are briefly discussed and recommendations are made for the preferred choice of sequences to obtain rapidly artifact-free data. This review covers diffusion experiments (DOSY), HSQC and HMBC experiments, ultra-resolved 2D spectra exploiting the property of aliasing and NOESY/ROESY experiments. It will be in particular shown that selective 1D NOESY/ROESY sequences can be more informative and reach higher resolution in less experimental time than the corresponding 2D sequences.