Shrinkage-mediated imaging of entire organs and organisms using uDISCO
Shrinkage-mediated imaging of entire organs and organisms using uDISCO
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DOI：
10.1038/nmeth.3964
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发表时间：
2016-10-01
期刊：
影响因子：
48
通讯作者：
Ertuerk, Ali
中科院分区：
文献类型：
--
作者：
Pan, Chenchen;Cai, Ruiyao;Ertuerk, Ali
近期的组织透明化方法已成为标准组织学方法的重要替代方案。然而，厚组织中的光散射以及标准光片显微镜可成像样本的尺寸限制，对分析诸如整个啮齿动物身体这样的大样本造成了局限。我们开发了“终极数字扫描激光光片层照显微镜成像兼容的组织透明化方法（uDISCO）”来克服体积成像中的这些限制。uDISCO可使荧光蛋白保存数月，使完整的器官和啮齿动物身体透明，同时使其尺寸缩小多达65%。我们利用uDISCO对超过7厘米从头到脚的神经连接和脉管系统进行成像，并对成年小鼠整个身体内移植的干细胞进行无偏筛选。uDISCO与多种标记方法以及存档的人体组织兼容，并且它可轻易用于各种生物医学应用中，以研究整个生物体中大型器官系统的组织结构。
Recent tissue-clearing approaches have become important alternatives to standard histology approaches. However, light scattering in thick tissues and the size restrictions on samples that can be imaged with standard light-sheet microscopy pose limitations for analyzing large samples such as an entire rodent body. We developed 'ultimate DISCO' (uDISCO) clearing to overcome these limitations in volumetric imaging. uDISCO preserves fluorescent proteins over months and renders intact organs and rodent bodies transparent while reducing their size up to 65%. We used uDISCO to image neuronal connections and vasculature from head to toe over 7 cm and to perform unbiased screening of transplanted stem cells within the entire body of adult mice. uDISCO is compatible with diverse labeling methods and archival human tissue, and it can readily be used in various biomedical applications to study organization of large organ systems throughout entire organisms.