Hemorrhage-induced vasopressin release in the paraventricular nucleus measured by in vivo microdialysis.
Hemorrhage-induced vasopressin release in the paraventricular nucleus measured by in vivo microdialysis.
复制标题
通过体内微透析测量出血引起的室旁核中加压素的释放。
DOI：
10.1016/s0006-8993(09)90009-9
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发表时间：
1994
期刊：
影响因子：
2.9
通讯作者：
Share,L
中科院分区：
文献类型：
--
作者：
Ota,M;Crofton,JT;Share,L
进行了实验，在清醒的大鼠体内微透析技术，以确定是否出血，一个强有力的刺激释放加压素从垂体后叶进入循环，也会导致局部释放加压素从室旁核（PVN），以及这种释放是否受性别的影响。在实验前一天，在PVN和股动脉和静脉导管附近使用微透析探针准备雄性和非发情雌性大鼠。在实验当天，对大鼠放血20%或30%的血容量。透析液中加压素的浓度在两次透析后的雄性和30%出血后的雌性中显著增加。出血后透析液加压素浓度在性别或出血程度方面无统计学显著差异。血浆血管加压素浓度显着增加，对出血的反应，这种反应是在30%出血后的女性更大。两种出血后动脉压降低无性别差异。它的结论是，生理刺激后叶加压素释放到循环中也导致下丘脑内释放这种激素。
Experiments were carried out, using the technique of in vivo microdialysis in conscious rats, to determine whether hemorrhage, a potent stimulus for the release of vasopressin from the posterior pituitary into the circulation, would also result in a local release of vasopressin from the paraventricular nucleus (PVN), and whether this release is affected by gender. Male and non-estrous female rats were prepared with a microdialysis probe adjacent to the PVN and femoral arterial and venous catheters the day before the experiment. On the day of the experiment, rats was bled either 20% or 30% of blood volume. The concentration of vasopressin in the dialysate increased significantly in the males following both hemorrhages and in the females following the 30% hemorrhage. There were no statistically significant differences in the post-hemorrhage dialysate vasopressin concentration with respect to either gender or magnitude of the hemorrhage. The plasma vasopressin concentration increased markedly in response to the hemorrhage and this response was greater in females following the 30% hemorrhage. There were no gender differences in the reduction in arterial pressure following either hemorrhage. It is concluded that physiological stimuli for the release of vasopressin into the circulation also result in intrahypothalamic release of this hormone.