The heat shock cognate protein 70 is associated with hepatitis C virus particles and modulates virus infectivity.
The heat shock cognate protein 70 is associated with hepatitis C virus particles and modulates virus infectivity.
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DOI：
10.1002/hep.22852
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发表时间：
2009-06
期刊：
影响因子：
--
通讯作者：
Beretta L
中科院分区：
文献类型：
--
作者：
Parent R;Qu X;Petit MA;Beretta L
There is growing evidence that virus particles contain host cell proteins. These proteins may provide viruses with means to evade the immune system or with mechanisms for cell entry and release. A proteomic analysis carried out on highly purified HCV J6/JFH virions identified the heat shock cognate protein 70 (HSC70) as part of the viral particles. These results were further validated by immunogold electron microscopy. The HSC70 interaction HPD motif was found present on the E2 envelope of the J6/JFH strain, as well as in over 50% of genotype 2 clinical HCV isolates. In addition, HSC70 was found associated with viral particles from an HCV genotype 2a-infected patient. Pre-incubation of HCV particles with anti-HSC70 antibodies decreased viral infectivity. Within infected cells, colocalization of HSC70 with the HCV core and E2 proteins was observed around lipid droplets. Reduction of HSC70 expression by an RNAi approach decreased the volume of lipid droplets as well as viral release without affecting HCV replication levels. These results suggest that HSC70 modulates HCV infectivity and lipid-droplet dependent virus release.
