Detection of circulating pancreas epithelial cells in patients with pancreatic cystic lesions.
Detection of circulating pancreas epithelial cells in patients with pancreatic cystic lesions.
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DOI：
10.1053/j.gastro.2013.12.007
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发表时间：
2014-03
期刊：
影响因子：
29.4
通讯作者：
Stanger BZ
中科院分区：
文献类型：
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作者：
Rhim AD;Thege FI;Santana SM;Lannin TB;Saha TN;Tsai S;Maggs LR;Kochman ML;Ginsberg GG;Lieb JG;Chandrasekhara V;Drebin JA;Ahmad N;Yang YX;Kirby BJ;Stanger BZ
血行播散被认为是癌症进展的晚期事件。我们最近发现，在胰腺导管腺癌（PDAC）的遗传模型中，可以在肿瘤形成前的血流中检测到胰腺细胞。为了在人类中证实这些发现，我们使用微流体几何增强免疫捕获来检测患者血液样本中的循环胰腺上皮细胞（CEC）。我们在21例囊性病变和无癌症临床诊断（仙台标准阴性）的患者中的7例（33%）、11例PDAC患者中的8例（73%）和19例无囊肿或癌症的患者中的0例（对照）中捕获了>3 CEC/ml。这些发现表明，癌细胞在肿瘤发生之前就存在于患者的循环中，这可能用于风险评估。
Hematogenous dissemination is thought to be a late event in cancer progression. We showed recently that pancreas cells can be detected in the bloodstream before tumor formation, in a genetic model of pancreatic ductal adenocarcinoma (PDAC). To confirm these findings in humans, we used microfluidic geometrically enhanced immunocapture to detect circulating pancreas epithelial cells (CECs) in patient blood samples. We captured >3 CECs/ml in 7 of 21 (33%) of patients with cystic lesions and no clinical diagnosis of cancer (Sendai criteria negative), 8 of 11 (73%) with PDAC, and in 0 of 19 patients without cysts or cancer (controls). These findings indicate that cancer cells are present in the circulation of patients before tumors develop, which might be used in risk assessment.