The distribution of ganglioside-like moieties in peripheral nerves.
The distribution of ganglioside-like moieties in peripheral nerves.
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神经节苷脂样部分在周围神经中的分布。
DOI：
10.1093/brain/122.3.449
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Griffin,JW
中科院分区：
文献类型：
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作者：
Sheikh,KA;Deerinck,TJ;Ellisman,MH;Griffin,JW
GM1神经节苷脂在一些周围神经系统疾病中被认为是免疫攻击的靶标。抗gm1神经节苷脂抗体与某些获得性免疫介导的神经病变有关。目前尚不清楚抗gm1抗体如何引起神经功能障碍和损伤；然而，Ranvier结的钠和/或钾离子通道功能障碍也有牵连。为了深入了解这些神经病变的发病机制，我们检测了神经纤维中GM1神经节苷脂和gal (β1-3) galnacy的分布，以及它们与人类、大鼠和营养不良小鼠周围神经Ranvier节点上电压门控钠和钾（Kv1.1, 1.5）通道的关系。Gal（β1-3）通过霍乱毒素和花生凝集素的结合定位。作为这些发现特异性的对照，我们比较了GM1和神经节苷脂GT1b的分布。我们的研究为成熟髓鞘纤维和雪旺细胞的腋膜表面存在含gal （β1-3）的部分提供了明确的证据。Gal(β1-3) galna结合位点与电压门控钠离子通道或钾离子通道Kv1.1和Kv1.5没有强制性的共定位，因此不可能由这些离子通道携带。与gal (β1-3)GalNAc相反，gt1b样部分仅限于轴膜。
GM1 ganglioside has been implicated as a target of immune attack in some diseases of the peripheral nervous system. Anti-GM1 ganglioside antibodies are associated with certain acquired immune-mediated neuropathies. It is not clear how anti-GM1 antibodies cause nerve dysfunction and injury; however, sodium and/or potassium ion channel dysfunction at the node of Ranvier has been implicated. To gain insight into the pathogenesis of these neuropathies, we examined the distribution of GM1 ganglioside andGal(β1–3)GalNAcmoieties in nerve fibres and their relationship to voltage-gated sodium and potassium (Kv1.1, 1.5) channels at the nodes of Ranvier in peripheral nerves from human, rat and dystrophic mice.Gal(β1–3)GalNAcmoieties were localized via the binding of cholera toxin and peanut agglutinin. As a control for the specificity of these findings, we compared the distribution of GM1 moieties to that of the ganglioside GT1b. Our study provides definitive evidence for the presence ofGal(β1–3)GalNAcbearing moieties on the axolemmal surface of mature myelinated fibres and on Schwann cells.Gal(β1–3)GalNAcbinding sites did not have an obligatory co-localization with voltage-gated sodium channels or the potassium ion channels Kv1.1 and Kv1.5 and are thus not likely carried by these ion channels. In contrast withGal(β1–3)GalNAc,GT1b-like moieties are restricted to the axolemma.