PHYLOGENY AND PHENOTYPIC CHARACTERIZATION OF THE STALK-FORMING AND IRON-OXIDIZING BACTERIUM GALLIONELLA-FERRUGINEA
PHYLOGENY AND PHENOTYPIC CHARACTERIZATION OF THE STALK-FORMING AND IRON-OXIDIZING BACTERIUM GALLIONELLA-FERRUGINEA
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DOI：
10.1099/00221287-139-7-1531
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发表时间：
1993-07-01
期刊：
影响因子：
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通讯作者：
PEDERSEN, K
中科院分区：
文献类型：
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作者：
HALLBECK, L;STAHL, F;PEDERSEN, K
采用聚合酶链反应（PCR）技术扩增了铜绿假单胞菌（Gallionella ferrugulosa）的16 S rRNA基因，并进行了直接双链测序。系统发育分析将G.在变形菌门的β群中，与多形亚硝化菌（Nitrosolobus multiformis）的相似性为90.0%，与紫红红环菌（Rhodocyclus purpureus）的相似性为88.6%。对已发表的G.用亚铁、硫代硫酸盐和硫化物作为电子供体，用硝酸盐作为氮源，编制了ferruglets，并补充了生长实验。G.铁锈菌是一种革兰氏阴性弯曲细菌，具有一根极鞭毛。它以CO2、葡萄糖、果糖和蔗糖为碳源，亚铁为电子供体，铵或硝酸盐为氮源，自养和混养生长。两千提出了铁蛋白酶特异性寡核苷酸探针。一株铁氧化细菌，不具有形成茎杆的能力，但具有与G. ferrugelae，鉴定为G.通过比较16 S rRNA基因的高度可变部分来确定铁蛋白。这表明茎对于生长不是必需的。
The 16S rRNA gene of Gallionella ferruginea was amplified by polymerase chain reaction and sequenced by direct double-stranded sequencing. The phylogenetic analysis placed G. ferruginea in the beta-group of the Proteobacteria, with 90.0% similarity to Nitrosolobus multiformis and 88.6% to Rhodocyclus purpureus. The published phenotypic characteristics of G. ferruginea were compiled and supplemented with growth experiments using ferrous iron, thiosulphate and sulphide as electron donor, and nitrate as nitrogen source. G. ferruginea is a Gram-negative, curved bacterium with one polar flagellum. It grows auto- and mixotrophically with CO2, glucose, fructose and sucrose as carbon sources, ferrous iron as an electron donor and ammonium or nitrate as nitrogen sources. Two G. ferruginea specific oligonucleotide probes are suggested. An iron-oxidizing bacterium without stalk-forming ability, but with the same growth pattern as G. ferruginea, was identified as G. ferruginea by comparison of highly variable parts of the 16S rRNA gene. This indicates that the stalk is not essential for growth.