Single nanoparticle trapping using a Raman tweezers microscope
Single nanoparticle trapping using a Raman tweezers microscope
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DOI：
10.1366/0003702021955015
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发表时间：
2002-04-01
影响因子：
3.5
通讯作者：
Torimitsu, K
中科院分区：
文献类型：
--
作者：
Ajito, K;Torimitsu, K
我们首次获得了被激光镊子捕获的单个纳米颗粒(纳米颗粒)的拉曼光谱。这项研究中使用的显微镜是我们的拉曼镊子显微镜(RTM)的新版本；它包含一个浸油的物镜，具有高数值孔径，以增加近红外激光束的光辐射压力的力，以捕获单个有机和生物纳米颗粒，并为捕获的纳米颗粒的拉曼测量提供足够的灵敏度。系统中的共焦布置完全消除了物镜下油的拉曼信号。RTM提供了直径约40 nm、曝光时间为3 S的单个聚苯乙烯乳胶珠的拉曼光谱，并使我们能够确定其分子物种。此外，RTM使我们能够从捕获的纳米粒子的拉曼光谱中计算出激光焦点中的纳米粒子的数量。
We have obtained a Raman spectrum of single nanometer-sized particles (nano-particles) trapped by laser tweezers for the first time. The microscope used in this study is a new version of our Raman tweezers microscope (RTM); it contains an oil-immersed objective lens with high numerical aperture to increase the force of the optical radiation pressure of the near-infrared laser beam to trap single organic and biological nanoparticles and provides sufficient sensitivity for the Raman measurement of the trapped nanoparticles. The confocal arrangement in the system completely eliminates the Raman signal of the oil under the objective lens. The RTM provides a Raman spectrum of a single poly styrene latex bead about 40 nm in diameter with an exposure time of 3 s and allows us to determine its molecular species. Furthermore, the RTM enables us to count the number of nanoparticles in the focal spot of the laser beam from the Raman spectra of trapped nanoparticles.