Response of rat osteoblast-like cells to microstructured model surfaces in vitro
Response of rat osteoblast-like cells to microstructured model surfaces in vitro
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DOI：
10.1016/s0142-9612(02)00379-4
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发表时间：
2003-02-01
期刊：
影响因子：
14
通讯作者：
Thomsen, P
中科院分区：
文献类型：
--
作者：
Liao, HH;Andersson, AS;Thomsen, P
研究了表面微形貌结合不同表面润湿性对大鼠颅细胞分化的影响。比较了亲水型和疏水型大鼠颅细胞中扁平聚二甲基硅氧烷（PDMS）和含金字塔型PDMS的矿化和碱性磷酸酶（ALP）活性。ALP表达细胞在含有金字塔的亲水PDMS上更为常见。ALP活性在第9天达到峰值，亲水性金字塔次之，亲水性金字塔次之，亲水性PDMS平面表面次之。矿化结核也有类似的规律。这些观察结果表明，微尺寸的表面特征促进了大鼠颅细胞的分化。此外，与疏水表面相比，亲水表面更容易刺激分化。结果表明，材料表面化学和形貌均影响成骨细胞的分化。(C) 2002 Elsevier Science Ltd.版权所有。
The role of surface microtopography in combination with different surface wettability for rat calvaria cell differentiation was examined. Mineralization and alkaline phosphatase (ALP) activity of rat calvaria cells oil flat polydimethylsiloxane (PDMS) or PDMS contained pyramids which were either hydrophilic or hydrophobic were compared. ALP expressing cells were more frequent on hydrophilic PDMS contained pyramids. ALP activity, peaked at day 9, was highest for hydrophilic pyramids followed by hydrophobic pyramids and flat hydrophilic PDMS surfaces. A similar pattern was obtained with respect to mineralized nodules. These observations showed that micro-sized surface features promote differentiation of rat calvaria cells. Further, hydrophilic Surfaces are more prone to stimulate differentiation in comparison with hydrophobic surfaces. The results suggest that both material surface chemistry and topography affect osteoblast differentiation. (C) 2002 Elsevier Science Ltd. All rights reserved.