Characterization of a novel influenza a virus hemagglutinin subtype (H16) obtained from black-headed gulls
Characterization of a novel influenza a virus hemagglutinin subtype (H16) obtained from black-headed gulls
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DOI：
10.1128/jvi.79.5.2814-2822.2005
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发表时间：
2005-03-01
影响因子：
5.4
通讯作者：
Osterhaus, ADME
中科院分区：
文献类型：
--
作者：
Fouchier, RAM;Munster, V;Osterhaus, ADME
在世界各地的野生水禽和家禽中，已描述了携带15种血凝素（HA）抗原亚型和9种神经氨酸酶（NA）抗原亚型的甲型流感病毒。在这里，我们描述了一个以前未确定的抗原亚型HA（1116），检测到的病毒在瑞典的黑头鸥循环。与抗原亚型定义的既定标准一致，血凝抑制试验和免疫扩散试验未能检测到H16与先前描述的亚型H1至H15之间的特异性反应性。在遗传学上，H16 HA被发现是远亲H13 HA，亚型也只在水鸟中检测到，和H13和H16 HA的推定受体结合位点的氨基酸组成被发现是不同的RA亚型在鸭和鹅循环。H16病毒含有NA基因，与其他欧亚水鸟的NA基因相似，但与在其他鸟类和地理位置检测到的N3基因在遗传上不同。欧洲海鸥病毒根据其PB2、NP和NS基因与其他甲型流感病毒进一步区分。获得关于A型禽流感病毒全谱的信息并开发用于检测和鉴定的试剂仍将是流感监测、疫情控制以及动物和公共卫生的一项重要任务。我们建议，HA和NA基因的序列分析甲型流感病毒用于快速鉴定现有的和新的HA和NA亚型。
In wild aquatic birds and poultry around the world, influenza A viruses carrying 15 antigenic subtypes of hemagglutinin (HA) and 9 antigenic subtypes of neuraminidase (NA) have been described. Here we describe a previously unidentified antigenic subtype of HA (1116), detected in viruses circulating in black-headed gulls in Sweden. In agreement with established criteria for the definition of antigenic subtypes, hemagglutination inhibition assays and immunodiffusion assays failed to detect specific reactivity between H16 and the previously described subtypes H1 to H15. Genetically, H16 HA was found to be distantly related to H13 HA, a subtype also detected exclusively in shorebirds, and the amino acid composition of the putative receptorbinding site of H13 and H16 HAs was found to be distinct from that in RA subtypes circulating in ducks and geese. The H16 viruses contained NA genes that were similar to those of other Eurasian shorebirds but genetically distinct from N3 genes detected in other birds and geographical locations. The European gull viruses were further distinguishable from other influenza A viruses based on their PB2, NP, and NS genes. Gaining information on the full spectrum of avian influenza A viruses and creating reagents for their detection and identification will remain an important task for influenza surveillance, outbreak control, and animal and public health. We propose that sequence analyses of HA and NA genes of influenza A viruses be used for the rapid identification of existing and novel HA and NA subtypes.