Transplantation of cultured salivary gland cells into an atrophic salivary gland
Transplantation of cultured salivary gland cells into an atrophic salivary gland
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DOI：
10.3727/000000004783983567
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发表时间：
2004-01-01
影响因子：
3.3
通讯作者：
Ueda, M
中科院分区：
文献类型：
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作者：
Sugito, T;Kagami, H;Ueda, M
口干患者已使用唾液替代品和/或药物（如毛果芸香碱或盐酸西维美林）进行治疗。这些治疗暂时缓解了他们的症状，并从残留的组织中诱导唾液分泌。然而，没有治疗可用于再生萎缩的腺体的目的。在这项研究中，细胞移植治疗萎缩的大鼠下颌下腺的可行性进行了研究。此外，通过免疫组织化学以及用荧光染料PKH 26进行的细胞追踪来评估细胞分化成有用表型的潜力。切除大鼠下颌下腺，并将唾液腺上皮细胞与3 T3细胞作为饲养层培养3周。结扎下颌下腺导管以产生萎缩的腺体。术后1周，去除结扎，将PKH 26标记的培养细胞注射到萎缩的下颌下腺内。作为对照，将培养的细胞也注射到正常下颌下腺中。细胞移植后两周，实验组和对照组中均可检测到移植细胞。细胞聚集在小叶之间的结缔组织中。移植后4周，实验组可检测到标记细胞，而对照组则未检测到。在萎缩的腺体中，观察到散在的移植细胞遍布腺体的广泛区域，但主要局限于腺泡和导管区域。免疫组化结果显示，移植细胞可能参与了导管再生，而移植后4周内既没有观察到肌上皮细胞也没有观察到腺泡分化。这项研究表明，细胞移植到唾液腺是可行的，并且移植的细胞被选择性地吸引并保留在受损区域而不影响正常组织。
Patients with dry mouth have been treated with salivary substitutes and/or medications such as pilocarpine or cevimeline hydrochloride. These treatments temporarily relieve their symptoms and induce salivation from residual tissue. However, no treatment is available for the purpose of regenerating an atrophic gland. In this study, the feasibility of a cell transplantation therapy for the atrophic submandibular glands was investigated in rats. Further, the potential of cell differentiation into a useful phenotype was assessed by immunohistochemistry together with cell tracking with the fluorescent dye PKH 26. Rat submandibular glands were excised, and the salivary gland epithelial cells were cultured for 3 weeks with 3T3 cells as a feeder layer. Ductal ligation of the submandibular gland was employed to generate an atrophic gland. One week after the operation, the ligation was removed, and the cultured cells labeled with PKH 26 were injected into the atrophic submandibular glands. As a control, the cultured cells were also injected into normal submandibular glands. Two weeks after cell transplantation, the transplanted cells were detectable in both the experimental and control groups. The cells were clustered in the connective tissue between the lobules. Four weeks after transplantation, the labeled cells were detectable in the experimental group but not in the control group. In the atrophic glands, the scattered transplanted cells were observed over a broad area of the gland but localized mainly around the acini and ductal region. Immunostaining results showed a possible involvement of the transplanted cells in ductal regeneration, while neither myoepithelial nor acinar differentiations were observed within the 4 weeks since transplantation. This study demonstrated that cell transplantation to the salivary gland is feasible, and that the transplanted cells were selectively attracted to and remained in the damaged area without affecting normal tissue.