Molecular cloning of a plasma membrane aquaporin in Stipa purpurea, and exploration of its role in drought stress tolerance
Molecular cloning of a plasma membrane aquaporin in Stipa purpurea, and exploration of its role in drought stress tolerance
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紫花针茅质膜水通道蛋白的分子克隆及其耐旱作用探讨
DOI：
10.1016/j.gene.2018.04.056
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发表时间：
2018-07-30
期刊：
影响因子：
3.5
通讯作者：
Yang, Yongping
中科院分区：
文献类型：
--
作者：
Chen, Qian;Yang, Shihai;Yang, Yongping
紫花针茅广泛分布于青藏高原，具有较高的抗旱性。质膜内在蛋白是一类水通道蛋白。它们调节水的运动，并与植物对生物和非生物胁迫的保护反应有关。我们鉴定了一种来自S. purpurea（SpPIP1），并阐明了其在植物干旱胁迫响应的分子方面的作用。SpPIP1基因的开放阅读框全长870 bp，编码289个氨基酸。SpPIP1基因在根中的转录水平较高。紫花苜蓿的花、叶和茎中的含量高于紫花苜蓿。干旱处理显著提高了SpPIP1的转录水平。亚细胞定位结果显示SpPIP1定位于质膜。SpPIP1在拟南芥中的异位表达导致植物对干旱处理具有更高的耐受性。SpPIP1为S.紫花苜蓿可能介导植物对干旱环境的响应。
Stipa purpurea is widely distributed on the Tibetan Plateau, and has high drought resistance. Plasma membrane intrinsic proteins are a type of aquaporin. They regulate the movement of water and are associated with plant protective reactions to biotic and abiotic stresses. We characterized a plasma membrane intrinsic protein from S. purpurea (SpPIP1) and elucidated its role in molecular aspects of the plant's response to drought stress. The full-length open reading frame of SpPIP1 was 870 bp and encoded 289 amino acids. The transcript level of SpPIP1 was higher in the root of S. purpurea than in the flower, leaf and stem. The level of SpPIP1 transcript increased significantly when treated with drought treatment. Subcellular localization result showed that SpPIP1 was localized in the plasma membrane. Ectopic expression of SpPIP1 in Arabidopsis thaliana resulted in plants with higher tolerance to drought treatment. SpPIP1 of S. purpurea may mediate plant response to arid environments.