The effects of silorane composites on levels of cytokines and periodontal parameters.
The effects of silorane composites on levels of cytokines and periodontal parameters.
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DOI：
10.4103/0976-237x.123021
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发表时间：
2013-10
影响因子：
1.2
通讯作者：
Kiziltunç A
中科院分区：
文献类型：
--
作者：
Ilday NO;Celik N;Dilsiz A;Alp HH;Aydin T;Seven N;Kiziltunç A
本研究的目的是确定硅硼烷复合材料对龈沟液（GCF）中肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）和IL-8水平、GCF体积和牙周临床参数的影响。共20名全身健康的非吸烟者，12名女性和8名男性（年龄范围：24-46岁），患有25例龈下边缘原发性牙本质龋。获得了大学伦理委员会的批准，治疗计划也得到了患者的批准。用牙周膜条从相关牙齿获得GCF样品用于IL-6、IL-8和TNF-α的测量。每个样品都储存在-80 °C下，并使用酶联免疫吸附测定（ELISA）试剂盒进行分析。根据粘合剂固化的一般原理制备空洞，并用硅烷粘合剂系统（硅烷系统粘合剂（3 M ESPE）和硅烷复合材料（Filtek Silorane，3 M ESPE））修复。恢复治疗后2周重新评估细胞因子水平。在α =0.05的显著性水平下，使用独立t检验分析数据。使用Pearson相关分析来分析参数之间的关联。治疗15天后，牙龈指数（GI）和菌斑指数（PI）均显著增加（P < 0.05）。龈沟液体积、IL-6、IL-8和TNF-α水平在修复治疗前后差异有统计学意义（P < 0.05）。除PI/GCF体积和GI/GCF体积外，其他参数之间均呈强正相关。在本研究的限制范围内，硅烷复合材料可能对细胞因子水平、临床参数和GCF体积具有一些负面影响。
The purpose of this pilot study was to determine the effects of silorane composites on gingival crevicular fluid (GCF) levels of tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6) and IL-8, GCF volume and clinical periodontal parameters in patients with silorane composite restorations before and after restorative treatment. A total of 20 systemically healthy non-smokers, 12 female and 8 male (age range: 24-46 years), presenting with 25 instances of primary dentine caries with subgingival margins were selected for this study. Approval was obtained from the university ethics committee and treatment plans were approved by the patients. GCF samples were obtained with periopaper strips from relevant teeth for IL-6, IL-8 and TNF-α measurements. Each sample was stored at − 80°C and analyzed using the enzyme-linked immunosorbent assay (ELISA) kits. Cavities were prepared according to the common principles for adhesive restorations and restored with a silorane adhesive system (Silorane System Adhesive (3M ESPE) and silorane composite (Filtek Silorane, 3M ESPE). Cytokine levels were reassessed 2 weeks after restorative treatment. Data were analyzed using the independent t-test at a significance level of α =0.05. Associations between parameters were analyzed using Pearson correlation analysis. A significant increase in gingival index (GI) and plaque index (PI) were observed after 15 days (P < 0.05). GCF volume, IL-6, IL-8 and TNF-α levels exhibited significant differences before and after restorative treatment (P < 0.05). There were strong positive correlations among parameters except for PI/GCF volume and GI/GCF volume. Within the limitations of this investigation, silorane composites may have some negative effects on cytokine levels, clinical parameters and GCF volume.