Multi-parent advanced generation inter-cross (MAGIC) populations in rice: progress and potential for genetics research and breeding.
Multi-parent advanced generation inter-cross (MAGIC) populations in rice: progress and potential for genetics research and breeding.
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DOI：
10.1186/1939-8433-6-11
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发表时间：
2013-05-06
期刊：
影响因子：
--
通讯作者：
Leung H
中科院分区：
文献类型：
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作者：
Bandillo N;Raghavan C;Muyco PA;Sevilla MA;Lobina IT;Dilla-Ermita CJ;Tung CW;McCouch S;Thomson M;Mauleon R;Singh RK;Gregorio G;Redoña E;Leung H
本文介绍了水稻多亲本高世代间杂交群体（MAGIC）的发展，并讨论了其在数量性状位点（QTL）定位和水稻品种开发中的潜在应用。我们已经开发了4个多亲本群体：籼稻MAGIC（8个籼稻亲本）; MAGIC plus（8个籼稻亲本，再进行两轮8向F1互交）;粳稻MAGIC（8个粳稻亲本）;和Global MAGIC（16个亲本- 8个籼稻和8个粳稻）。用于创建这些群体的亲本是具有生物和非生物胁迫耐受性、产量和谷物品质的理想性状的改良品种。目的是对多个性状的QTL进行精细定位，并直接或间接地利用高度重组系进行育种。这些MAGIC群体提供了一个有用的种质资源，具有不同的等位基因组合，可供水稻社区利用。籼稻MAGIC群体是迄今为止开发的最先进的MAGIC群体，包括通过单种子血统（SSD）产生的1328个品系。在S4阶段的SSD的一个子集（200行），这个群体的基因型，使用基因分型测序（GBS）的方法和表型的多个性状，包括：稻瘟病和白叶枯病抗性，耐盐性和耐淹性，和谷物品质。全基因组关联定位确定了几个已知的主效基因和QTL，包括与耐淹性相关的Sub1和与白叶枯病抗性相关的Xa 4和xa 5。此外，全基因组关联研究（GWAS）的结果还发现了与水稻改良的基本性状相关的潜在新位点。MAGIC群体具有双重目的：用于精确QTL作图的永久作图群体以及用于品种开发的直接和间接用途。与一组自然多样的种质不同，该群体是为育种者量身定制的，具有来自多个精英育种系的有用性状的组合。MAGIC种群也为研究基因组渗入和染色体重组的相互作用提供了机会。本文的在线版本（doi：10.1186/1939-8433-6-11）包含补充材料，可供授权用户使用。
This article describes the development of Multi-parent Advanced Generation Inter-Cross populations (MAGIC) in rice and discusses potential applications for mapping quantitative trait loci (QTLs) and for rice varietal development. We have developed 4 multi-parent populations: indica MAGIC (8 indica parents); MAGIC plus (8 indica parents with two additional rounds of 8-way F1 inter-crossing); japonica MAGIC (8 japonica parents); and Global MAGIC (16 parents – 8 indica and 8 japonica). The parents used in creating these populations are improved varieties with desirable traits for biotic and abiotic stress tolerance, yield, and grain quality. The purpose is to fine map QTLs for multiple traits and to directly and indirectly use the highly recombined lines in breeding programs. These MAGIC populations provide a useful germplasm resource with diverse allelic combinations to be exploited by the rice community. The indica MAGIC population is the most advanced of the MAGIC populations developed thus far and comprises 1328 lines produced by single seed descent (SSD). At the S4 stage of SSD a subset (200 lines) of this population was genotyped using a genotyping-by-sequencing (GBS) approach and was phenotyped for multiple traits, including: blast and bacterial blight resistance, salinity and submergence tolerance, and grain quality. Genome-wide association mapping identified several known major genes and QTLs including Sub1 associated with submergence tolerance and Xa4 and xa5 associated with resistance to bacterial blight. Moreover, the genome-wide association study (GWAS) results also identified potentially novel loci associated with essential traits for rice improvement. The MAGIC populations serve a dual purpose: permanent mapping populations for precise QTL mapping and for direct and indirect use in variety development. Unlike a set of naturally diverse germplasm, this population is tailor-made for breeders with a combination of useful traits derived from multiple elite breeding lines. The MAGIC populations also present opportunities for studying the interactions of genome introgressions and chromosomal recombination. The online version of this article (doi:10.1186/1939-8433-6-11) contains supplementary material, which is available to authorized users.