EPIDERMAL GROWTH-FACTOR STIMULATES THE GROWTH OF A431 TUMORS IN ATHYMIC MICE
EPIDERMAL GROWTH-FACTOR STIMULATES THE GROWTH OF A431 TUMORS IN ATHYMIC MICE
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DOI：
10.1016/0304-3835(85)90069-2
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发表时间：
1985-01-01
期刊：
影响因子：
9.7
通讯作者：
VONDERHAAR, BK
中科院分区：
文献类型：
--
作者：
GINSBURG, E;VONDERHAAR, BK
体外人表皮样癌细胞系A431的生长受到低浓度表皮生长因子（EGF; 0.1-10 pM）的刺激，并受到高浓度（0.1-10 nM）的抑制。这种细胞在雌性无胸腺小鼠中也以实体瘤的形式生长。体内持续高水平的EGF可以通过皮下插入基于胆固醇的颗粒向雌性小鼠施用睾酮来实现。然而，EGF水平的这种慢性升高（血清浓度= 90 ng/ml）并不影响肿瘤的生长。相反，低水平的生长因子（0.5 μ g/g体重，注射5次/周;血清浓度= 8.25 ng/ml）刺激肿瘤生长。这些数据表明，EGF在体外抑制A431细胞生长的机制在体内不起作用，EGF在体内的生理学显著作用是促进生长。
Growth of the human epidermoid carcinoma cell line A431 in vitro in stimulated by low concentrations of epidermal growth factor (EGF; 0.1-10 pM) and inhibited by high concentrations (0.1-10 nM). This cell also grows as a solid tumor in female athymic mice. Sustained high levels of EGF in vivo can be achieved by the administration of testosterone to female mice via a cholesterol-based pellet inserted subcutaneously. This chronic elevation of EGF levels (serum concentration = 90 ng/ml), however, does not affect growth of the tumor. In contrast, low levels of the growth factor (0.5 .mu.g/g body wt by injection 5 times/week; serum concentration = 8.25 ng/ml) stimulate growth of the tumor. These data suggest that the mechanism(s) involved in the inhibition of A431 cell growth by EGF in vitro does not function in vivo and the physiologically significant effect of EGF in vivo is growth promotion.