Comparison of the effects of marchantin C and fucoidan on sFlt-1 and angiogenesis in glioma microenvironment
Comparison of the effects of marchantin C and fucoidan on sFlt-1 and angiogenesis in glioma microenvironment
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DOI：
10.1111/j.2042-7158.2011.01430.x
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发表时间：
2012-04-01
影响因子：
3.3
通讯作者：
Li, Xingang
中科院分区：
文献类型：
--
作者：
Lv, Yafeng;Song, Qingxu;Li, Xingang
目的研究马钱子素C和岩藻糖胶对胶质瘤细胞和单核细胞诱导血管生成的影响，并探讨sFlt-1在此过程中的作用。条件培养液用于内皮细胞管形成试验和sFlt-1的酶联免疫吸附试验。通过中和抗体去除sFlt-1，以评估其在此过程中的作用。关键发现Marchantin C抑制T98G细胞诱导的血管生成，而岩藻糖胶抑制T98G和THP1细胞诱导的血管生成。在血管生成受到抑制的三组中，培养上清液中sFlt-1水平均升高。经sFlt-1抗体处理的条件培养液可在一定程度上恢复抑制的血管生成。结论首次提出马钱子素和岩藻糖胶对胶质瘤细胞或单核细胞诱导的血管生成有明显的抑制作用。SFlt-1的上调在这一过程中发挥了重要作用。
Objectives This study aimed to examine the effects of marchantin C and fucoidan on angiogenesis induced by glioma cells and monocytes, and to elucidate the role of sFlt-1 in this process.Methods T98G glioma cells and THP1 monocytes were pretreated with marchantin C or fucoidan, respectively. Conditioned media were used for endothelial cell tube formation assay and detection of sFlt-1 by ELISA. Depletion of sFlt-1 was achieved by a neutralizing antibody to assess its role in the process.Key findings Marchantin C inhibited angiogenesis induced by T98G cells while fucoidan inhibited both T98G and THP1 cell-induced angiogenesis. In all three groups in which angiogenesis was inhibited, sFlt-1 level in the supernatants was elevated. Pretreatment of the conditioned media with sFlt-1 antibody restored the inhibited angiogenesis to a certain degree.Conclusions This study suggested for the first time that marchantinCand fucoidan could significantly inhibit angiogenesis induced by glioma cells or monocytes. Up-regulation of sFlt-1 played an important role in this process.