Venomics of the spider Ornithoctonus huwena based on transcriptomic versus proteomic analysis
Venomics of the spider Ornithoctonus huwena based on transcriptomic versus proteomic analysis
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基于转录组与蛋白质组分析的虎纹鸟蛛毒液组学
DOI：
10.1016/j.cbd.2010.01.001
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发表时间：
2010-06-01
影响因子：
3
通讯作者：
Liang, Songping
中科院分区：
文献类型：
--
作者：
Jiang, Liping;Zhang, Dongyi;Liang, Songping
虎纹捕鸟蛛（Ornithoctonus huwena）是一种产于中国南方的毒蜘蛛。它的毒液是由许多生物活性成分组成的复杂混合物。在这项研究中，41个新的独特的转录本编码细胞蛋白质或其他可能的毒液成分，从先前构建的cDNA文库中产生。这些蛋白质也被注释的KOG（真核生物直链组）和GO（基因本体论）术语。发现了一个新的编码EF-手蛋白的细胞转录重叠群（命名为HWEFHP 1），该蛋白可能参与毒腺毒素的分泌。为了对O.胡文纳毒液，迄今为止已知的所有转录本，肽和蛋白质的数据集进行了分析。通过蛋白质组学与转录组学方法获得的数据的比较显示，只有15个推定的胱氨酸结毒素（CKTs）被确定的两种方法，29个转录编码CKTs被发现在转录组中，但不是作为翻译的肽在毒液蛋白质组中。然而，两种方法均未鉴定出具有相同分子量的细胞蛋白。我们的数据可能有助于更深入地了解O. huwena和个别毒素的结构和功能之间的关系。(C)2010年爱思唯尔公司All rights reserved.
The spider Ornithoctonus huwena is a venomous spider found in southern China. Its venom is a complex mixture of numerous biologically active components. In this study, 41 novel unique transcripts encoding cellular proteins or other possible venom components were generated from the previously constructed cDNA library. These proteins were also annotated by KOG (eukaryotic orthologous group) and GO (gene ontology) terms. A novel cellular transcript contig encoding an EF-hand protein (named HWEFHP1) was found, which might be involved in the secretion of toxins in the venom glands. In order to have an overview of the molecular diversity of the O. huwena venom, the datasets of all the transcripts, peptides and proteins known so far were analyzed. A comparison of the data obtained through a proteomic versus a transcriptomic approach, revealed that only 15 putative cystine knot toxins (CKTs) were identified by both approaches, 29 transcripts coding for CKTs were found in the transcriptome but not as translated peptides in the venom proteome. However, no cellular protein with identical molecular weight was identified by both approaches. Our data may contribute to a deeper understanding of the biology and ecology of O. huwena and the relationship between structure and function of individual toxins. (C) 2010 Elsevier Inc. All rights reserved.