Staphylococcus aureus invasion of bovine mammary epithelial cells
Staphylococcus aureus invasion of bovine mammary epithelial cells
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DOI：
10.3168/jds.s0022-0302(96)76454-8
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发表时间：
1996-06-01
影响因子：
3.5
通讯作者：
Oliver, SP
中科院分区：
文献类型：
--
作者：
Almeida, RA;Matthews, KR;Oliver, SP
金黄色葡萄球菌是奶牛乳房炎的常见原因。然而，感染的发病机制尚未完全确定。我们报告了两株S.金黄色葡萄球菌转化为牛乳腺上皮细胞系和牛乳腺上皮细胞原代培养物。入侵S。金黄色葡萄球菌进入牛乳腺细胞是时间依赖性的。用透射电镜观察S.金黄色葡萄球菌显示细菌在膜结合空泡内的细胞内复制。当牛乳腺上皮细胞用F-肌动蛋白微丝聚合的抑制剂处理时，侵袭显着减少，但当这些细胞用微管形成的抑制剂处理时，侵袭显着减少。结果表明，S.金黄色葡萄球菌能够在牛乳腺上皮细胞内侵入和复制。数据还表明，S.金黄色葡萄球菌侵入牛乳腺上皮细胞需要上皮细胞细胞骨架的特定组分的积极参与。
Staphylococcus aureus is a frequent cause of mastitis in dairy cows. However, pathogenesis of the infection has not been completely defined. We report the invasion of two strains of S. aureus into a bovine mammary epithelial cell line and a bovine mammary epithelial cell primary culture. Invasion of S. aureus into bovine mammary cells was time-dependent. Transmission electron microscopy of bovine mammary cells invaded by S. aureus showed intracellular replication of the bacterium within membrane-bound vacuoles. Invasion was reduced significantly when bovine mammary epithelial cells were treated with inhibitors of F-actin microfilament polymerization but not when these cells were treated with inhibitors of microtubule formation. Results indicated that S. aureus is capable of invading and replicating inside bovine mammary epithelial cells. Data also suggested that S. aureus invasion of bovine mammary epithelial cells requires active participation of specific components of the cytoskeleton of the epithelial cell.