In Vitro Cow's Milk Protein-Specific Inflammatory and Regulatory Cytokine Responses in Preterm Infants With Necrotizing Enterocolitis and Sepsis
In Vitro Cow's Milk Protein-Specific Inflammatory and Regulatory Cytokine Responses in Preterm Infants With Necrotizing Enterocolitis and Sepsis
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DOI：
10.1203/pdr.0b013e31820263e7
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发表时间：
2011-02-01
影响因子：
3.6
通讯作者：
Fell, John M. E.
中科院分区：
文献类型：
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作者：
Abdelhamid, Adel E.;Chuang, Shu-Ling;Fell, John M. E.
牛奶配方肠内喂养与新生儿坏死性小肠结肠炎（NEC）和败血症有关。在这两种情况下，饮食抗原致敏可能在促进和/或维持炎症方面发挥作用。为了研究NEC和脓毒症早产儿的牛奶蛋白（CMP）特异性细胞因子反应，我们招募了14名NEC患儿、14名匹配的健康对照组和10名脓毒症对照组。采用单细胞酶联免疫斑点法（ELISPOT）检测未刺激和刺激的外周血单核细胞（PBMCs）分泌ifn - γ、IL-4、IL-10和tgf - β 1的数量。在NEC的急性期，患者在未刺激和有丝分裂原[植物血凝素（PHA）]和CMPs: β -乳球蛋白（β -lg）和酪蛋白刺激下均表现出高水平的细胞因子分泌的一般模式。与tgf - β 1相比，ifn - γ、IL-4和IL-10对β -lg的反应更为明显。脓毒症患者的细胞因子反应低于NEC患者（健康对照组最低，tgf - β 1反应最小）。在足月，细胞因子分泌细胞的频率比急性期低，但tgf - β 1分泌细胞除外，其在足月增加（对PHA和CMPs的反应），特别是在NEC和败血症之后。（儿科研究69:165-169,2011）
Enteral feeding with cow's milk formula is associated with neonatal necrotizing enterocolitis (NEC) and sepsis. Dietary antigen sensitization may play a role in promoting and/or sustaining inflammation in both conditions. Aiming at investigating cow's milk protein (CMP)-specific cytokine responses in preterm infants with NEC and sepsis, 14 babies with NEC, 14 matched healthy controls, and 10 septic controls were recruited. Unstimulated and stimulated peripheral blood mononuclear cells (PBMCs) secreting IFN-gamma, IL-4, IL-10, and TGF-beta 1 were counted by the single-cell enzyme-linked immunospot (ELISPOT) assay. During the acute phase of NEC, patients showed a general pattern of a high level of cytokine secretion both when unstimulated and stimulated by mitogen [phytohaemagglutinin (PHA)] and CMPs: beta-lactoglobulin (beta-lg) and casein. These responses were more marked to beta-lg for IFN-gamma, IL-4, and IL-10 than TGF-beta 1. Cytokine responses in sepsis were lower than in NEC (lowest in healthy controls, with a minimal TGF-beta 1 response). At term, lower frequencies of cytokine-secreting cells were elicited than during the acute phase, except for TGF-beta 1 secreting cells, which increased at term (in response to PHA and CMPs) particularly following not only NEC but also sepsis. (Pediatr Res 69: 165-169, 2011)