Analysis of electron spin resonance spectra of alkyl spin labels in excised guinea pig dorsal skin, its stratum corneum, delipidized skin and stratum corneum model lipid liposomes.
Analysis of electron spin resonance spectra of alkyl spin labels in excised guinea pig dorsal skin, its stratum corneum, delipidized skin and stratum corneum model lipid liposomes.
复制标题
离体豚鼠背部皮肤、角质层、脱脂皮肤和角质层模型脂质脂质体中烷基自旋标记的电子自旋共振谱分析。
DOI：
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发表时间：
2001
影响因子：
1.7
通讯作者：
A. Ikarashi
中科院分区：
文献类型：
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作者：
S. Kitagawa;A. Ikarashi
在豚鼠切除背侧皮肤、角质层、脱皮皮肤和角质层模型脂质体中观察烷基自旋标记的电子自旋共振（ESR）谱。角质层中5-羟基硬脂酸（5-NS）的光谱和有序参数表明，自旋标记存在于高度有序的脂质片层中。另一方面，5-NS甲酯（5-NMS）的光谱及其表观旋转相关时间显示，在角质层和整个切除皮肤中只有弱固定化成分。酯自旋标签似乎几乎不存在于刚性脂质片层中，而主要存在于相对流动的环境中。另一方面，不同的烷基链长度，阳离子烷基自旋标记的光谱有很大的不同。正如我们最近报道的那样，长链4-（N，N-二甲基-N，-pentadecyl）铵-2,2,6,6-四甲基哌啶-1-氧（CAT-15）似乎存在于角质层的蛋白质区域，而亲水季铵自旋标记4-三甲基铵-2,2,6,6-四甲基哌啶-1-氧（CAT-1）似乎存在于皮肤的大部分水中，即使在脱水的皮肤中也是如此。这些结果表明，烷基自旋标记的不同相互作用和不同定位取决于它们的电子电荷和烷基链长度。
The electron spin resonance (ESR) spectra of alkyl spin labels were observed in the excised guinea pig dorsal skin, its stratum corneum, delipidized skin and stratum corneum model lipid liposomes. The spectrum of 5-doxylstearic acid (5-NS) in the stratum corneum and order parameter obtained from the spectrum, indicated that the spin label was present in highly ordered lipid lamella. On the other hand, the spectrum of methyl ester of 5-NS (5-NMS) and its apparent rotational correlation time calculated from the spectrum, showed only a weakly immobilized component in the stratum corneum as well as in the whole excised skin. The ester spin label seemed to be scarcely present in the rigid lipid lamella, but mainly in the relatively fluid environment. On the other hand, cationic alkyl spin labels showed quite different spectra depending on their alkyl chain lengths. Long-chain 4-(N,N-dimethyl-N,-pentadecyl)ammonium-2,2,6,6-tetramethylpiperidine-1-oxyl (CAT-15) seemed to be present in the protein region of the stratum corneum as we recently reported, whereas hydrophilic quaternary ammonium spin label 4-trimethylammonium-2,2,6,6-tetramethylpiperidine-1-oxyl (CAT-1) seemed to be present in the bulk water of the skin, even in delipidized skin. These findings indicated that the different interaction and different localization of the alkyl spin labels depended on their electronic charge as well as their alkyl chain lengths.