Tracing the temporal-spatial transcriptome landscapes of the human fetal digestive tract using single-cell RNA-sequencing
Tracing the temporal-spatial transcriptome landscapes of the human fetal digestive tract using single-cell RNA-sequencing
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DOI：
10.1038/s41556-018-0105-4
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发表时间：
2018-06-01
影响因子：
21.3
通讯作者：
Tang, Fuchou
中科院分区：
文献类型：
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作者：
Gao, Shuai;Yan, Liying;Tang, Fuchou
消化道的发育对于适当的食物消化和营养吸收至关重要。在这里，我们分析了消化道的主要器官，包括食道，胃，小肠和大肠，来自妊娠6至25周的人类胚胎以及成年人的大肠，使用单细胞RNA-seq分析。总共分析了5，227个单个细胞，并明确识别了40种细胞类型。系统地揭示了它们的重要生物学特征，包括发育过程、信号通路、细胞周期、营养消化和吸收代谢以及转录因子网络。此外，大肠的分化和成熟过程进行了彻底的研究，通过比较胚胎和成人阶段之间的相应的转录组谱。我们的工作为在单细胞分辨率下研究人胎儿消化道和成人大肠的基因调控网络提供了丰富的资源。
The development of the digestive tract is critical for proper food digestion and nutrient absorption. Here, we analyse the main organs of the digestive tract, including the oesophagus, stomach, small intestine and large intestine, from human embryos between 6 and 25 weeks of gestation as well as the large intestine from adults using single-cell RNA-seq analyses. In total, 5,227 individual cells are analysed and 40 cell types clearly identified. Their crucial biological features, including developmental processes, signalling pathways, cell cycle, nutrient digestion and absorption metabolism, and transcription factor networks, are systematically revealed. Moreover, the differentiation and maturation processes of the large intestine are thoroughly investigated by comparing the corresponding transcriptome profiles between embryonic and adult stages. Our work offers a rich resource for investigating the gene regulation networks of the human fetal digestive tract and adult large intestine at single-cell resolution.