Effect of Yuzu (Citrus junos) Seed Limonoids and Spermine on Intestinal Microbiota and Hypothalamic Tissue in the Sandhoff Disease Mouse Model.
Effect of Yuzu (Citrus junos) Seed Limonoids and Spermine on Intestinal Microbiota and Hypothalamic Tissue in the Sandhoff Disease Mouse Model.
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DOI：
10.3390/medsci9010017
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发表时间：
2021-03-11
期刊：
影响因子：
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通讯作者：
Yamanaka S
中科院分区：
文献类型：
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作者：
Minamisawa M;Suzumura T;Bose S;Taniai T;Kawai G;Suzuki K;Yamaguchi A;Yamanaka S
研究了柚子籽中提取的柠檬素和精胺（Spm）对Sandhoff病（SD）小鼠肠道和大脑的影响。野生型和SD型小鼠分别饲喂正常饲粮、添加类柠檬素、Spm或类柠檬素+ Spm饲粮14-18周，提取粪便DNA进行16S rRNA基因扩增子测序。对于SD对照小鼠，肠道微生物群主要由乳酸杆菌组成，并与生态失调有关。在SD小鼠和野生型小鼠中，单独饲喂柠檬素+ Spm或单独饲喂柠檬素，肠道微生物群中含有丰富的黏液降解菌，包括拟杆菌门、Verrucomicrobia和厚壁菌门，并且显示出更高的短链脂肪酸和免疫球蛋白a的产生，并且柠檬素+ Spm或单独饲喂柠檬素的SD小鼠下丘脑组织炎症更少，神经元数量更多。给药柠檬素和/或Spm可改善SD小鼠有益肠道微生物群对宿主健康的比例，并减少神经元变性。柚子类柠檬素和精胺可能有助于维持SD小鼠肠道微生物群和下丘脑组织的稳态。
The effect of limonoids and spermine (Spm) extracted from yuzu (Citrus junos) seeds on the gut and the brain in a mouse model with Sandhoff disease (SD) was investigated. Wild-type and SD mice were fed a normal diet, or a diet supplemented with limonoid, Spm, or limonoid + Spm for 14–18 weeks, and then 16S rRNA gene amplicon sequencing with extracted DNA from their feces was executed. For SD control mice, intestinal microbiota was mostly composed of Lactobacillus and linked to dysbiosis. For SD and wild-type mice fed with limonoids + Spm or limonoids alone, intestinal microbiota was rich in mucin-degrading bacteria, including Bacteroidetes, Verrucomicrobia, and Firmicutes, and displayed a higher production of short-chain fatty acids and immunoglobulin A. Additionally, SD mice fed with limonoids + Spm or limonoids alone had less inflammation in hypothalamic tissues and displayed a greater number of neurons. Administration of limonoids and/or Spm improved the proportions of beneficial intestinal microbiota to host health and reduced neuronal degeneration in SD mice. Yuzu seed limonoids and Spermine may help to maintain the homeostasis of intestinal microbiota and hypothalamic tissue in the SD mouse model.