Unannotated Open Reading Frame in Saccharomyces cerevisiae Encodes Protein Localizing to the Endoplasmic Reticulum.
Unannotated Open Reading Frame in Saccharomyces cerevisiae Encodes Protein Localizing to the Endoplasmic Reticulum.
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DOI：
10.17912/micropub.biology.000992
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发表时间：
2023
影响因子：
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通讯作者：
Carvunis, Anne-Ruxandra
中科院分区：
文献类型：
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作者：
Chang, Scott;Joyson, Matthew;Kelly, Anna;Tang, Lucas;Iannotta, John;Rich, April;Castilho Coelho, Nelson;Carvunis, Anne-Ruxandra
酿酒酵母基因组中有数千个未注释的翻译开放阅读框架(ORF)。以前对一个这样的未注释的ORF的研究表明，用AAG取代ORF的ATG起始密码子会导致细胞在不同的应激条件下适应能力的变化(Wachold等人，2023)。这表明YGR016C-A的翻译在细胞适合性中起作用。在这里，我们研究Ygr016c-a的亚细胞定位，以了解其细胞功能。计算预测工具、共表达分析和荧光显微镜显示，Ygr016c-a蛋白定位于内质网。
There are thousands of unannotated translated open reading frames (ORFs) in the Saccharomyces cerevisiae genome. Previous investigation into one such unannotated ORF, which was systemically labeled YGR016C-A based on its genomic coordinates, showed that replacing the ORF’s ATG start codon with AAG led to a change in cellular fitness under different stress conditions (Wacholder et al., 2023). This suggested translation of YGR016C-A plays a role in cellular fitness. Here, we investigate Ygr016c-a’s subcellular localization to gain insight into its cellular function. Computational prediction tools, co-expression analysis and fluorescence microscopy suggest that the Ygr016c-a protein localizes to the endoplasmic reticulum.